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Efficient utilization of cassava pulp for succinate production by metabolically engineered Escherichia coli KJ122

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Abstract

A metabolically engineered Escherichia coli KJ122 was efficiently utilized for succinate production from cassava pulp during batch separate hydrolysis and fermentation (SHF) under simple anaerobic conditions. Succinate concentration of 41.46 ± 0.05 g/L with yield and productivity of 82.33 ± 0.14 g/100 g dry pulp and 0.84 ± 0.02 g/L/h was obtained. In batch simultaneous saccharification and fermentation (SSF), hydrolysis of 12 % (w/v) cassava pulp with an enzyme loading of 2 % AMG + 3 % Cel (v/w) at pH 6.5 was optimized at 39 °C. Succinate concentration of 80.86 ± 0.49 g/L with a yield of 70.34 ± 0.37 g/100 g dry pulp and a productivity of 0.84 ± 0.01 g/L/h was attained using E. coli KJ122. Fed-batch SSF significantly enhanced succinate concentration to 98.63 ± 0.12 g/L at yield and productivity of 71.64 ± 0.97 g/100 g dry pulp and 1.03 ± 0.01 g/L/h. This result indicated an efficient and economical succinate production from cassava pulp using SHF and SSF by the use of E. coli KJ122.

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Acknowledgments

This work was financially supported by the Thailand Research Fund (TRF) under the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0135/2553). The authors thank Professor Lonnie Ingram who cordially provided E. coli KJ122 used in this study. Thanks to Bob Tremayne, Ubon Ratchathani University Office of International Relations for assistance with English.

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Authors and Affiliations

  1. Metabolic Engineering Research Unit, Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-District, Muang District, Nakhon Ratchasima, 30000, Thailand

    Apichai Sawisit, Sunthorn Kanchanatawee & Kaemwich Jantama

  2. Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani, 34190, Thailand

    Sirima Suvarnakuta Jantama

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  1. Apichai Sawisit
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  2. Sirima Suvarnakuta Jantama
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Correspondence to Kaemwich Jantama.

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Sawisit, A., Jantama, S.S., Kanchanatawee, S. et al. Efficient utilization of cassava pulp for succinate production by metabolically engineered Escherichia coli KJ122. Bioprocess Biosyst Eng 38, 175–187 (2015). https://doi.org/10.1007/s00449-014-1257-7

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  • DOI: https://doi.org/10.1007/s00449-014-1257-7

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