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Production of lactic acid and ethanol by Rhizopus oryzae integrated with cassava pulp hydrolysis

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Abstract

Cassava pulp was hydrolyzed with acids or enzymes. A high glucose concentration (>100 g/L) was obtained from the hydrolysis with 1 N HCl at 121 °C, 15 min or with cellulase and amylases. While a high glucose yield (>0.85 g/g dry pulp) was obtained from the hydrolysis with HCl, enzymatic hydrolysis yielded only 0.4 g glucose/g dry pulp. These hydrolysates were used as the carbon source in fermentation by Rhizopus oryzae NRRL395. R. oryzae could not grow in media containing the hydrolysates treated with 1.5 N H2SO4 or 2 N H3PO4, but no significant growth inhibition was found with the hydrolysates from HCl (1 N) and enzyme treatments. Higher ethanol yield and productivity were observed from fermentation with the hydrolysates when compared with those from fermentation with glucose in which lactic acid was the main product. This was because the extra organic nitrogen in the hydrolysates promoted cell growth and ethanol production.

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Acknowledgments

This work has been financially supported by Commission on Higher Education and Thailand Research Fund (Grant number: MRG5080097). Raw cassava pulp was kindly provided by Chonjareon Cassava Mill, Thailand. We thank Sitanan Thitiprasert for assistance in analyzing fermentation samples and Siam Victory Chemicals, Thailand, for technical supports and providing enzyme samples (Genencor products).

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Authors and Affiliations

  1. Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Institute Building 3, Phayathai Road, Wangmai, Pathumwan, Bangkok, 10330, Thailand

    Nuttha Thongchul & Surapong Navankasattusas

  2. Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH, 43210, USA

    Shang-Tian Yang

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  1. Nuttha Thongchul
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  2. Surapong Navankasattusas
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  3. Shang-Tian Yang
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Correspondence to Nuttha Thongchul.

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Thongchul, N., Navankasattusas, S. & Yang, ST. Production of lactic acid and ethanol by Rhizopus oryzae integrated with cassava pulp hydrolysis. Bioprocess Biosyst Eng 33, 407–416 (2010). https://doi.org/10.1007/s00449-009-0341-x

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