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Swainsonine, a novel fungal metabolite: optimization of fermentative production and bioreactor operations using evolutionary programming

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Abstract

The optimization of bioreactor operations towards swainsonine production was performed using an artificial neural network coupled evolutionary program (EP)-based optimization algorithm fitted with experimental one-factor-at-a-time (OFAT) results. The effects of varying agitation (300–500 rpm) and aeration (0.5–2.0 vvm) rates for different incubation hours (72–108 h) were evaluated in bench top bioreactor. Prominent scale-up parameters, gassed power per unit volume (P g/V L, W/m3) and volumetric oxygen mass transfer coefficient (K L a, s−1) were correlated with optimized conditions. A maximum of 6.59 ± 0.10 μg/mL of swainsonine production was observed at 400 rpm-1.5 vvm at 84 h in OFAT experiments with corresponding P g/VL and K L a values of 91.66 W/m3 and 341.48 × 10−4 s−1, respectively. The EP optimization algorithm predicted a maximum of 10.08 μg/mL of swainsonine at 325.47 rpm, 1.99 vvm and 80.75 h against the experimental production of 7.93 ± 0.52 μg/mL at constant K L a (349.25 × 10−4 s−1) and significantly reduced P g/V L (33.33 W/m3) drawn by the impellers.

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Abbreviations

OFAT:

One-factor-at-a-time

EP:

Evolutionary programming/evolutionary program

N :

Agitation rate (rpm)

Q :

Aeration rate (vvm)

P g :

Gassed power requirement (W)

P g/V L :

Gassed power required per unit volume (W/m3)

K L a :

Volumetric oxygen mass transfer coefficient (s−1)

F g :

Gas flow rate (m3/s)

V g :

Gas superficial velocity (m/s)

W i :

Impeller blade width (m)

N i :

Rotations per second (rps)

g :

Acceleration due to gravity (m/s2)

D i :

Impellers diameter (m)

D t :

Tank diameter (m)

τ av :

Average shear stress (N/m2)

γ av :

Average shear rate (s−1)

µ a :

Apparent viscosity according to Ostwald de-Waele model (Pa s)

µ g :

Gas viscosity (Pa s)

η eff :

Eddy length (m)

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Acknowledgments

We thank Indian Institute of Technology Guwahati for providing the experimental facilities and Council of Scientific and Industrial research (CSIR), PUSA, New Delhi, India, for providing the research fellowship. The authors have no conflict of interest.

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  1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India

    Digar Singh & Gurvinder Kaur

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  1. Digar Singh
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  2. Gurvinder Kaur
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Correspondence to Gurvinder Kaur.

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Singh, D., Kaur, G. Swainsonine, a novel fungal metabolite: optimization of fermentative production and bioreactor operations using evolutionary programming. Bioprocess Biosyst Eng 37, 1599–1607 (2014). https://doi.org/10.1007/s00449-014-1132-6

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