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Optimization, production and characterization of glycolipid biosurfactant from the marine actinobacterium, Streptomyces sp. MAB36

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Abstract

A potential glycolipid biosurfactant producer Streptomyces sp. MAB36 was isolated from marine sediment samples. Medium composition and culture conditions for the glycolipid biosurfactant production by Streptomyces sp. MAB36 were optimized, using two statistical methods: Plackett–Burman design was applied to find out the key ingredients and conditions for the best yield of glycolipid biosurfactant production and central composite design was used to optimize the concentration of the four significant variables, starch, casein, crude oil and incubation time. Fructose and yeast extract were the best carbon and nitrogen sources for the production of the glycolipid biosurfactant. Biochemical characterizations including FTIR and MS studies suggested the glycolipid nature of the biosurfactant. The isolated glycolipid biosurfactant reduced the surface tension of water from 73.2 to 32.4 mN/m. The purified glycolipid biosurfactant showed critical micelle concentrations of 36 mg/l. The glycolipid biosurfactant was effective at very low concentrations over a wide range of temperature, pH, and NaCl concentration. The purified glycolipid biosurfactant showed strong antimicrobial activity. Thus, the strain Streptomyces sp. MAB36 has proved to be a potential source of glycolipid biosurfactant that could be used for the bioremediation processes in the marine environment.

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Acknowledgments

This research was supported by a grant from Marine Bioprocess Research Center of the Marine Biotechnology Program funded by the Ministry of Oceans and Fisheries, Republic of Korea. One of the authors, Kannan Sivakumar expresses his thanks to the Director, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences and Annamalai University authorities for facilities and encouragement. Authors also thank Prof. L. Kannan, Former Vice-chancellor of Thiruvalluvar University, for critically going through the manuscript and offering comments.

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Authors and Affiliations

  1. Marine Biotechnology Laboratory, Department of Chemistry, Pukyong National University, Busan, 608-737, Republic of Korea

    Panchanathan Manivasagan, Jayachandran Venkatesan & Se-Kwon Kim

  2. Marine Bioprocess Research Center, Pukyong National University, Busan, 608-737, Republic of Korea

    Se-Kwon Kim

  3. Faculty of Marine Sciences, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, 608 502, Tamil Nadu, India

    Palaniappan Sivasankar & Kannan Sivakumar

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  1. Panchanathan Manivasagan
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  2. Palaniappan Sivasankar
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  3. Jayachandran Venkatesan
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Correspondence to Se-Kwon Kim.

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Manivasagan, P., Sivasankar, P., Venkatesan, J. et al. Optimization, production and characterization of glycolipid biosurfactant from the marine actinobacterium, Streptomyces sp. MAB36. Bioprocess Biosyst Eng 37, 783–797 (2014). https://doi.org/10.1007/s00449-013-1048-6

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