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The pH effects on the distribution of 1,3-propanediol and 2,3-butanediol produced simultaneously by using an isolated indigenous Klebsiella sp. Ana-WS5

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Abstract

Several carbon sources were investigated for the production of 1,3-propanediol (PDO) and 2,3-butanediol (BDO) simultaneously, using an isolated indigenous Klebsiella sp. Ana-WS5. The results indicate that glycerol is a suitable carbon source for both BDO and PDO production. Further investigation suggests that adjustment of the pH could alter the metabolic pathway, which affects the ratio of PDO and BDO obtained. The batch with pH controlled at 7.0 had the highest total diol (PDO + BDO) productivity of 0.86 g/L h and the highest PDO/BDO of 7.67, as compared to a batch with pH controlled at 6.0. However, the batch without pH control could achieve a maximum total diol concentration of 48.1 ± 1.6 g/L and the highest yield of 86 % (total diols produced/glycerol consumed). The effects of pH control on the distribution of PDO and BDO concluded in this study could be further applied to the process design for enhancing PDO or BDO production.

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Acknowledgments

The authors gratefully acknowledge financial support from Taiwan’s National Science Council.

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Authors and Affiliations

  1. Department of Chemical and Materials Engineering, Tunghai University, Taichung, 407, Taiwan

    Hong-Wei Yen & Fang-Tzu Li

  2. Department of Chemical Engineering, National Cheng Kung University, Tainan, 701, Taiwan

    Chiao-Ling Wong & Jo-Shu Chang

  3. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan, 701, Taiwan

    Jo-Shu Chang

  4. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, 701, Taiwan

    Jo-Shu Chang

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  1. Hong-Wei Yen
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  2. Fang-Tzu Li
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Correspondence to Hong-Wei Yen.

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Yen, HW., Li, FT., Wong, CL. et al. The pH effects on the distribution of 1,3-propanediol and 2,3-butanediol produced simultaneously by using an isolated indigenous Klebsiella sp. Ana-WS5. Bioprocess Biosyst Eng 37, 425–431 (2014). https://doi.org/10.1007/s00449-013-1008-1

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  • DOI: https://doi.org/10.1007/s00449-013-1008-1

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