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Improvement of human skin cell growth by radiation-induced modifications of a Ge/Ch/Ha scaffold

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Abstract

Gelatin-/chitosan-/hyaluronan-based biomaterials are used in tissue engineering as cell scaffolds. Three gamma radiation doses (1, 10 and 25 kGy) were applied to scaffolds for sterilization. Microstructural changes of the irradiated polymers were evaluated by using scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). A dose of 25 kGy produced a rough microstructure with a reduction of the porosity (from 99 to 96 %) and pore size (from 160 to 123 μm). Radiation also modified the glass transition temperature between 31.2 and 42.1 °C (1 and 25 kGy respectively). Human skin cells cultivated on scaffolds irradiated with 10 and 25 kGy proliferated at 48 h and secreted transforming growth factor β3 (TGF-β3). Doses of 0 kGy (non-irradiated) or 1 kGy did not stimulate TGF-β3 secretion or cell proliferation. The specific growth rate and lactate production increased proportionally to radiation dose. The use of an appropriate radiation dose improves the cell scaffold properties of biomaterials.

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Acknowledgments

The authors wish to thank CONICYT for the Fondecyt Grant (1110607) and FONDEF Grant (D07I1075) and Universidad Técnica Federico Santa María for the Piic2011 fellowship to Rodrigo A. Somoza.

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Authors and Affiliations

  1. Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile

    Cristian A. Acevedo, Rodrigo A. Somoza, Elizabeth Sánchez, Fernando Albornoz & Manuel E. Young

  2. Departamento de Bioquímica, Facultad de Farmacia, Universidad de Valparaíso, Avenida Gran Bretaña 1093, Valparaíso, Chile

    Caroline Weinstein-Oppenheimer

  3. Comisión Chilena de Energía Nuclear, Amunátegui 95, Santiago, Chile

    Samy Silva

  4. Departamento de Física, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile

    Macarena Moreno

  5. Division of Food Sciences, The University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK

    William MacNaughtan

  6. Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile, Avenida Ecuador 3769, Estación Central, Santiago, Chile

    Javier Enrione

Authors
  1. Cristian A. Acevedo
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  2. Rodrigo A. Somoza
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  3. Caroline Weinstein-Oppenheimer
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  4. Samy Silva
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  5. Macarena Moreno
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  6. Elizabeth Sánchez
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  7. Fernando Albornoz
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  8. Manuel E. Young
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  9. William MacNaughtan
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  10. Javier Enrione
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Corresponding author

Correspondence to Cristian A. Acevedo.

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Acevedo, C.A., Somoza, R.A., Weinstein-Oppenheimer, C. et al. Improvement of human skin cell growth by radiation-induced modifications of a Ge/Ch/Ha scaffold. Bioprocess Biosyst Eng 36, 317–324 (2013). https://doi.org/10.1007/s00449-012-0786-1

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  • DOI: https://doi.org/10.1007/s00449-012-0786-1

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