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Efficient conversion of high concentration of glycerol to Monacolin K by solid-state fermentation of Monascus purpureus using bagasse as carrier

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Abstract

High concentration of glycerol was used as the sole carbon source for efficient production of Monacolin K (MK) by solid-state fermentation (SSF) of Monascus purpureus 9901 using agricultural residue (bagasse), as an inert carrier. A comparative study showed that MK production in SSF was about 5.5 times higher than that of submerged fermentation when 26 % of glycerol was used, which may be due to the formation of glycerol concentration gradients in the inert carrier and less catabolite repression in SSF. For enhancement of MK yield in SSF, the effects of different influential variables, such as glycerol concentration, nitrogen source and its concentration, initial moisture content, inoculum size and particle size of bagasse, were systematically examined. All the factors mentioned above had an effect on the MK production in SSF to some extent. The maximal yield of MK (12.9 mg/g) was achieved with 26 % glycerol, 5 % soybean meal, 51 % initial moisture content, 20 % inoculum size and 1 mm particle size of bagasse. The results in this study may expand our understanding on the application of SSF using agricultural residue as carrier for production of useful microbial metabolites, especially the efficient conversion of high concentration of glycerol to MK by Monascus purpureus.

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Acknowledgments

We thank the National Natural Science Foundation of China (Grant No. 21046010), the Science Fund of Jiangnan University (Grant No. 573) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions for partial financial support.

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  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Li-Ping Lu, Bo-Bo Zhang & Gan-Rong Xu

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  1. Li-Ping Lu
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Correspondence to Gan-Rong Xu.

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Lu, LP., Zhang, BB. & Xu, GR. Efficient conversion of high concentration of glycerol to Monacolin K by solid-state fermentation of Monascus purpureus using bagasse as carrier. Bioprocess Biosyst Eng 36, 293–299 (2013). https://doi.org/10.1007/s00449-012-0784-3

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  • DOI: https://doi.org/10.1007/s00449-012-0784-3

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