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Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5

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Abstract

This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivity (5.19 mg/L/h) in glycerol-defined medium were 26.4% higher than the heparosan production (148 mg/L) and heparosan productivity (4.11 mg/L/h) in glucose-defined medium. DO-stat feeding approach as compared to pH-stat feeding, exponential feeding, exponential combined with pH-stat feeding, and constant rate feeding gave the highest heparosan titer at 8.63 g/L, which was nine times that of batch culture. The obtained optimal glycerol feeding strategy may be useful for the scaling-up of microbial heparosan production.

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Acknowledgments

This work was financially supported the Priority Academic Program Development of Jiangsu Higher Education Institutions, the National Natural Science Foundation of China (20836003), the Major State Basic Research Development Program of China (973 Program, 2012CB720806), and the National High Technology Research and Development Program of China (863 Program, 2011AA100905), and 111 Project (111-2-06).

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Authors and Affiliations

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China

    Yanfeng Liu, Long Liu, Jianghua Li, Guocheng Du & Jian Chen

  2. School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Yanfeng Liu, Long Liu, Jianghua Li & Guocheng Du

  3. School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, 214122, China

    Jinghua Chen

  4. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

    Jian Chen

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  1. Yanfeng Liu
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  2. Long Liu
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  3. Jinghua Chen
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  4. Jianghua Li
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  5. Guocheng Du
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Correspondence to Long Liu or Jian Chen.

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Liu, Y., Liu, L., Chen, J. et al. Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5. Bioprocess Biosyst Eng 35, 1209–1218 (2012). https://doi.org/10.1007/s00449-012-0708-2

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  • DOI: https://doi.org/10.1007/s00449-012-0708-2

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