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High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat

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An Erratum to this article was published on 29 April 2004

Abstract

A new feeding strategy in fed-batch culture, exponential feeding combined with pH-stat is suggested to avoid the accumulation of substrate in culture broth. Exponential feeding was stopped whenever a predetermined amount of limiting substrate was supplied and then pH change was observed. When pH rose above an upper limit due to the depletion of substrate, feeding was restarted. With this feeding strategy, recombinant Escherichia coli could be grown to 101 g/l by controlling the specific growth rate at 0.1 h−1.

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Authors and Affiliations

  1. Department of Chemical Engineering, Chungbuk National University, 361-763, Cheongju, Korea

    Beom Soo Kim, Seung Chul Lee, Sang Yup Lee, Yong Keun Chang & Ho Nam Chang

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  1. Beom Soo Kim
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  2. Seung Chul Lee
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  3. Sang Yup Lee
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  4. Yong Keun Chang
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  5. Ho Nam Chang
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Correspondence to Beom Soo Kim.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s00449-004-0361-5

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Kim, B.S., Lee, S.C., Lee, S.Y. et al. High cell density fed-batch cultivation of Escherichia coli using exponential feeding combined with pH-stat. Bioprocess Biosyst Eng 26, 147–150 (2004). https://doi.org/10.1007/s00449-003-0347-8

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  • DOI: https://doi.org/10.1007/s00449-003-0347-8

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