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Production and activities of chitinases and hydrophobins from Lecanicillium lecanii

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Abstract

The production of chitinases and hydrophobins from Lecanicillium lecanii was influenced by the cultivation method and type of carbon source. Crude enzyme obtained from solid-substrate culture presented activities of exochitinases (32 and 51 kDa), endochitinases (26 kDa), β-N-acetylhexosaminidases (61, 80, 96 and 111 kDa). Additionally, submerged cultures produced exochitinases (32 and 45 kDa), endochitinases (10 and 26 kDa) and β-N-acetylhexosaminidases (61, 96 and 111 kDa). β-N-acetylhexosaminidases activity determined in solid-substrate culture with added chitin was ca. threefold (7.58 ± 0.57 U mg−1) higher than submerged culture (2.73 + 0.57 U mg−1). Similarly, hydrophobins displayed higher activities in solid-substrate culture (627.3 ± 2 μg protein mL−1) than the submerged one (57.4 ± 4.7 μg protein mL−1). Molecular weight of hydrophobins produced in solid-substrate culture was 7.6 kDa and they displayed surface activity on Teflon.

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Acknowledgments

The authors gratefully acknowledged to CONACYT for the research funding (Project Number 105628) and for PhD scholarship grant (ZRP).

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Authors and Affiliations

  1. Laboratory of Biopolymers, Biotechnology Department, Universidad Autónoma Metropolitana, Av. San Rafael Atlixco, No. 186, 09340, Mexico City, Mexico

    Zaizy Rocha-Pino & Keiko Shirai

  2. Process and Technology Department, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico, D.F., Mexico

    Gabriel Vigueras

Authors
  1. Zaizy Rocha-Pino
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  2. Gabriel Vigueras
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  3. Keiko Shirai
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Correspondence to Keiko Shirai.

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Rocha-Pino, Z., Vigueras, G. & Shirai, K. Production and activities of chitinases and hydrophobins from Lecanicillium lecanii . Bioprocess Biosyst Eng 34, 681–686 (2011). https://doi.org/10.1007/s00449-011-0517-z

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