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Antibacterial functionalization of wool fabric via immobilizing lysozymes

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Abstract

Greater attention has been given to enzymatic processes of textiles as effective alternatives to conventional chemical treatments because of the non-toxic and eco-friendly characteristics of enzymes as well as the increasingly important requirement for reducing pollution in textile production. A new functionalization method for wool fabrics based on immobilization of lysozymes was investigated in this paper. Wool fabric was first activated with glutaraldehyde, and then employed to covalently immobilize lysozymes. Main immobilization parameters were optimized in terms of the activity of immobilized enzyme. A high activity of the immobilized enzyme was obtained when the fabric was activated at 25 °C for 6 h in a bath containing with 0.2% of glutaraldehyde followed by the immobilization at 4 °C and pH 7.0 for 6 h with 5 g l−1 lysozyme. The scanning electron microscopy and staining tests based on modified Coomassie protein assay (Bradford method) revealed that the lysozyme was fixed covalently on the wool fabric. Wool fabrics immobilizing lysozymes presented a higher ratio of bacteriostasis to Staphylococcus aureus. The durability of antibacterial wool was assessed and the lysozyme immobilized on wool fabric retained ca. 43% of its activity after five cycles of use when taking the activity of the immobilized lysozyme before using as reference.

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References

  1. Cavaco-Paulo A, Guebitz G (2003) Textile processing with enzymes. Woodhead Publishing, Cambridge, pp 107–130

    Google Scholar 

  2. Chen J, Wang Q, Hua ZZ, Dua GC (2006) Research and application of biotechnology in textile industries in China. Enzyme Microb Tech 40:1651–1655

    Article  Google Scholar 

  3. Schindler WD, Hauser PJ (2004) Chemical finishing of textiles. Woodhead Publishing, Cambridge, pp 165–174

    Google Scholar 

  4. Vigo TL (1983) Protection of textiles from biological attack. In: Lewin M, Sello SB (eds) Handbook of fiber science and technology, Vol. II, Chemical processing of fibers and fabrics, Functional finishes, Part A, Marcel Dekker, New York, pp 367–427

  5. Freedi G, Arai T (2001) Binding of metal cations to chemically modified wool and antimicrobial properties of the wool-metal complexes. J Appl Polym Sci 82:3513–3519

    Article  Google Scholar 

  6. Taddei P, Monti P, Freddi G, Arai T, Tsukada M (2003) Binding of Co(II) and Cu(II) cations to chemically modified wool fibres: an IR investigation. J Mol Struct 650:105–113

    Article  CAS  Google Scholar 

  7. Wang SH, Hou WS, Wei LQ, Jia HS, Liu XG, Xu BS (2007) Antibacterial activity of nano-SiO2 antibacterial agent grafted on wool surface. Surf Coat Tech 202:460–465

    Article  CAS  Google Scholar 

  8. Ki HY, Kim JH, Kwon SC, Jeong SH (2007) A study on multifunctional wool textiles treated with nano-sized silver. J Mater Sci 42:8020–8024

    Article  CAS  Google Scholar 

  9. Gao Y, Cranston R (2008) Recent advances in antimicrobial treatments of textiles. Text Res J 78:60–72

    Article  CAS  Google Scholar 

  10. Han SY, Yang YQ (2005) Antimicrobial activity of wool fabric treated with curcumin. Dyes Pigments 64:157–161

    Article  CAS  Google Scholar 

  11. Zhu P, Sun G (2004) Antimicrobial finishing of wool fabrics using quaternary ammonium salts. J Appl Polym Sci 93:1037–1041

    Article  CAS  Google Scholar 

  12. Purwar R, Joshi M (2004) Recent developments in antimicrobial finishing of textiles—a review. AATCC Rev 4:22–26

    CAS  Google Scholar 

  13. Pushpa B (2001) Finishing of textile materials. J Appl Polym Sci 83:631–659

    Google Scholar 

  14. Wikipedia, the free encyclopedia. http://en.wikipedia.org/wiki/Lysozyme. Accessed 1 Oct 2008

  15. Hughey VL, Johnson EA (1987) Antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease. Appl Environ Microbiol 53:2165–2170

    CAS  Google Scholar 

  16. Appendini P, Hotchkiss JH (1997) Immobilization of lysozyme on food contact. Polymers as potential antimicrobial films. Pack Technol Sci 10:271–279

    Article  CAS  Google Scholar 

  17. Ibrahim HR, Matsuzaki T, Aoki T (2001) Genetic evidence that antibacterial activity of lysozyme is independent of its catalytic function. FEBS Lett 506:27–32

    Article  CAS  Google Scholar 

  18. Zhao YP, Zhang H, Yang YJ (2002) Study on improvement of assaying lysozyme. Food Sci 23:116–119

    CAS  Google Scholar 

  19. Wang Q, Li CX, Fan XR, Wang P, Cui L (2008) Immobilization of catalase on cotton fabric oxidized by sodium periodate. Biocat Biotrans 26:437–443

    Article  CAS  Google Scholar 

  20. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  21. He MZ, Yan J, Gong Y (2005) Anti-bacterial fabric and the evaluation of its property. Shanghai Text Sci Tech 33:62–64

    Google Scholar 

  22. Guo Y (1994) Enzyme engineering. China light industry press, Beijing, pp 109–110

    Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National High Technology Research and Development Program of China (2008AA02Z203) and Jiangsu Provincial Undergraduate Innovation Project.

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Authors and Affiliations

  1. Key Laboratory of Science and Technology of Eco-Textile, Ministry of Education, Jiangnan University, Wuxi, Jiangsu, China

    Qiang Wang, Xuerong Fan, Yingjun Hu, Jiugang Yuan, Li Cui & Ping Wang

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  1. Qiang Wang
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  2. Xuerong Fan
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  3. Yingjun Hu
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  4. Jiugang Yuan
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  5. Li Cui
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  6. Ping Wang
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Correspondence to Xuerong Fan.

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Wang, Q., Fan, X., Hu, Y. et al. Antibacterial functionalization of wool fabric via immobilizing lysozymes. Bioprocess Biosyst Eng 32, 633–639 (2009). https://doi.org/10.1007/s00449-008-0286-5

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  • DOI: https://doi.org/10.1007/s00449-008-0286-5

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