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Lincomycin, rational selection of high producing strain and improved fermentation by amino acids supplementation

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Abstract

Based on the report that the introduction of the biosynthetic precursor of lincomycin, propylproline, could increase the production of lincomycin (Bruce et al. in US Patent 3,753,859, 1973), a mutant strain pro10–20, with resistance of feedback suppression of proline (an analog of propylproline) was thus selected and lincomycin production increased by 10%. The addition of three amino acids (l-proline, l-tyrosine, l-alanine) which are the precursors of propylproline to the fermentation medium was found to enhance the accumulation of l-dopa through different pathways and was favorable to lincomycin biosynthesis. The production of lincomycin was increased by 23, 10, 13%, respectively, with the addition of 0.05 g L−1 l-proline at 60 h, 0.005 g L−1 l-tyrosine and 0.1 g L−1 l-alanine directly in the medium.

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  1. Department of Bioengineering, East China University of Science and Technology, Shanghai, China

    Ruifang Ye, Qian Wang & Xiaofang Zhou

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  1. Ruifang Ye
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  2. Qian Wang
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  3. Xiaofang Zhou
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Correspondence to Ruifang Ye.

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Ye, R., Wang, Q. & Zhou, X. Lincomycin, rational selection of high producing strain and improved fermentation by amino acids supplementation. Bioprocess Biosyst Eng 32, 521–529 (2009). https://doi.org/10.1007/s00449-008-0272-y

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