Abstract
Candida cylindracea NRRL Y-17506 was grown to produce extracellular lipase from oleic acid as a carbon source. Through flask cultures, it was found that the optimum initial oleic acid concentration for cell growth was 20 g l−1. However, high initial concentrations of oleic acid up to 50 g l−1 were not inhibitory. The highest extracellular lipase activity obtained in flask culture was 3.0 U ml−1 after 48 h with 5 g l−1 of initial oleic acid concentration. Fed-batch cultures (intermittent and stepwise feeding) were carried out to improve cell concentration and lipase activity. For the intermittent feeding fed-batch culture, the final cell concentration was 52 g l−1 and the extracellular lipase activity was 6.3 U ml−1 at 138.5 h. Stepwise feeding fed-batch cultures were carried out to simulate an exponential feeding and to investigate the effects of specific growth rate (0.02, 0.04 and 0.08 h−1) on cell growth and lipase production. The highest final cell concentration obtained was 90 g l−1 when the set point of specific growth rate (μset) was 0.02 h−1. High specific growth rate (0.04 and 0.08 h−1) decreased extracellular lipase production in the later part of fed-batch cultures due to build-up of the oleic acid oversupplied. The highest extracellular lipase activity was 23.7 U ml−1 when μset was 0.02 h−1, while the highest lipase productivity was 0.31 U ml−1 h−1 at μset of 0.08 h−1.
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Acknowledgements
B.S. Kim would like to acknowledge the support of a Korea Research Foundation Grant (KRF-2005-013-D00015). The technical assistance of Ms. Amy Martinez and Mr. John Ludeman is highly appreciated. We thank C.P. Kurtzman of NCAUR, ARS, USDA for advice on the taxonomy of Candida cylindracea. The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over the firms or similar products not mentioned.
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Kim, B.S., Hou, C.T. Production of lipase by high cell density fed-batch culture of Candida cylindracea . Bioprocess Biosyst Eng 29, 59–64 (2006). https://doi.org/10.1007/s00449-006-0058-z
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DOI: https://doi.org/10.1007/s00449-006-0058-z