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Partial purification of α-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems

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Abstract

A study was made of the partition and purification of α-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)—citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of α-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of α-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride.

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Acknowledgements

The authors would like to thank the support from the Chinese “863” National High Technology Grant-2002AA217031.

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, People’s Republic of China

    Wenbo Zhi, Jiangnan Song, Jingxiu Bi & Fan Ouyang

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  1. Wenbo Zhi
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  2. Jiangnan Song
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  3. Jingxiu Bi
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  4. Fan Ouyang
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Correspondence to Wenbo Zhi.

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Zhi, W., Song, J., Bi, J. et al. Partial purification of α-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems. Bioprocess Biosyst Eng 27, 3–7 (2004). https://doi.org/10.1007/s00449-004-0369-x

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  • DOI: https://doi.org/10.1007/s00449-004-0369-x

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