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The role of vitamin D receptor in predentin mineralization and dental repair after injury

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Abstract

Dentin is a permeable and complex tubular composite formed by the mineralization of predentin that mineralization and repair are of considerable clinical interest during dentin homeostasis. The role of Vdr, a receptor of vitamin D, in dentin homeostasis remains unexplored. The aim of the present study was to assess the impact of Vdr on predentin mineralization and dental repair. Vdr-knockout (Vdr−/−) mice models were constructed; histology and immunohistochemistry analyses were conducted for both WT and Vdr−/− mice. The finding revealed a thicker predentin in Vdr−/− mice, characterized by higher expression of biglycan and decorin. A dental injury model was employed to observe tertiary dentin formation in Vdr−/− mice with dental injuries. Results showed that tertiary dentin was harder to form in Vdr−/− mice with dental injury. Over time, heightened pulp invasion was observed at the injury site in Vdr−/− mice. Expression of biglycan and decorin was reduced in the predentin at the injury site in the Vdr−/− mice by immunohistochemistry. Taken together, our results imply that Vdr plays a regulatory role in predentin mineralization and tertiary dentin formation during dentin homeostasis.

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The data used to support the findings of this study were included within the article.

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Acknowledgements

We thank Shanghai Model Organisms Center, Inc., generated Vdr-knockout mice. Thanks for editing this manuscript.

Funding

This work was supported by the Natural Science Fund of Education Department of Anhui Province [KJ2021A0817]; the University Synergy Innovation Program of Anhui Province, China [GXXT-2021-056]; and the Scientific Research Foundation of Education (Graduate Academic Innovation Project) Department of Anhui Province of China [2022xscx126].

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Author notes

  1. Yudong Liu and Yinlin Wu contributed equally to this work.

Authors and Affiliations

  1. Department of Histology and Embryology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, 233030, China

    Yudong Liu, Xiaodong Hu & Meiqun Sun

  2. Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, 233030, China

    Yudong Liu & Shanshan Liu

  3. Department of Stomatology, The First Affiliated Hospital of Bengbu Medical College, 287 Chuang Huai Road, Bengbu, 233004, China

    Shanshan Liu

  4. Department of Biochemistry and Molecular Biology, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, 233030, China

    Yu Sun

  5. Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, 233030, China

    Yinlin Wu, Guojin Zeng & Qinglong Wang

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  1. Yudong Liu
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Contributions

YDL, YLW, and MQS designed the research and wrote the manuscript. YS and SSL analyzed the data and reviewed the manuscript. XDH, GJZ, and QLW contributed to data collation and analysis. All authors reviewed the manuscript.

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Correspondence to Shanshan Liu or Meiqun Sun.

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All experiments involving animals were performed in accordance with the guidelines for the Care and Use of Laboratory Animals and monitored by the Institutional Animal Care and Use Committee of Bengbu Medical College (BBMC-2022-204).

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Liu, Y., Wu, Y., Hu, X. et al. The role of vitamin D receptor in predentin mineralization and dental repair after injury. Cell Tissue Res (2024). https://doi.org/10.1007/s00441-024-03886-7

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