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Targeted Disruption of Two Small Leucine-rich Proteoglycans, Biglycan and Decorin, Excerpts Divergent Effects on Enamel and Dentin Formation

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Abstract

Small leucine-rich proteoglycans have been suggested to affect mineralization of dental hard tissues. To determine the functions of two of these small proteoglycans during the early stages of tooth formation, we characterized the dental phenotypes of biglycan (BGN KO) and decorin deficient (DCN KO) mice and compared them to that of wild type mice. Each targeted gene disruption resulted in specific effects on dentin and enamel formation. Dentin was hypomineralized in both knock out mice, although the effect was more prominent in the absence of decorin. Enamel formation was dramatically increased in newborn biglycan knockout mice but delayed in absence of decorin. Increased enamel formation in the former case resulted from an upregulation of amelogenin synthesis whereas delayed enamel formation in the later case was most probably an indirect consequence of the high porosity of the underlying dentin. Enamelin expression was unchanged in BGN KO, and reduced in DCN KO. Dentin sialoprotein (DSP), a member of the family of phosphorylated extracellular matrix proteins that play a role in dentinogenesis, was overexpressed in BGN-KO odontoblasts and in the sub-odontoblastic layer. In contrast, a decreased expression of DSP was detected in DCN KO. Dentin matrix protein-1 (DMP-1), bone sialoprotein (BSP) and osteopontin (OPN) were upregulated in BGN KO and downregulated in the DCN KO. Despite the strong effects induced by these deficiencies in newborn mice, no significant difference was detected between the three genotypes in adult mice, suggesting that the effects reported here in newborn mice are transient and subjected to self-repair.

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Acknowledgments

We thank Dr. Karine Vidal for critical reading of the manuscript and Jennifer Clough for reviewing the English. We thanks also the Institute Benjamin Delessert for the grant kindly provided on “Proteoglycans and dental tissues”.

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Authors and Affiliations

  1. Laboratoire: Réparation et Remodelage des Tissus Oro-Faciaux, EA 2496, Groupe Matrices Extracellulaires et Minéralisations, Faculté de Chirurgie Dentaire, Université Paris V, Montrouge, 92120, France

    M. Goldberg, D. Septier & O. Rapoport

  2. Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA, 19107, USA

    R. V. Iozzo

  3. Craniofacial and Skeletal Diseases Branch, NIDCR, National Institute of Health, Bethesda Maryland, MD, 20892, USA

    M. F. Young & L. G. Ameye

  4. Department Nutrition and Health, Nestlé Research Center, 1000 Lausanne 26, Vers-chez-les-Blanc, P.O. Box 44, Switzerland

    L. G. Ameye

  5. Faculté de Chirurgie Dentaire -Université Paris V Matrices extracellulaires et biominéralisations, 1, rue Maurice Arnoux, Montrouge, 92120, France

    M. Goldberg

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  1. M. Goldberg
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  2. D. Septier
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  3. O. Rapoport
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  4. R. V. Iozzo
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  6. L. G. Ameye
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Correspondence to M. Goldberg.

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Goldberg, M., Septier, D., Rapoport, O. et al. Targeted Disruption of Two Small Leucine-rich Proteoglycans, Biglycan and Decorin, Excerpts Divergent Effects on Enamel and Dentin Formation. Calcif Tissue Int 77, 297–310 (2005). https://doi.org/10.1007/s00223-005-0026-7

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