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Quantitative proteomics of sperm tail in asthenozoospermic patients: exploring the molecular pathways affecting sperm motility

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Abstract

Asthenozoospermia, characterized by low sperm motility, is one of the most common causes of male infertility. While many intrinsic and extrinsic factors are involved in the etiology of asthenozoospermia, the molecular basis of this condition remains unclear. Since sperm motility results from a complex flagellar structure, an in-depth proteomic analysis of the sperm tail can uncover mechanisms underlying asthenozoospermia. This study quantified the proteomic profile of 40 asthenozoospermic sperm tails and 40 controls using TMT-LC–MS/MS. Overall, 2140 proteins were identified and quantified where 156 proteins have not been described earlier in sperm tail. There were 409 differentially expressed proteins (250 upregulated and 159 downregulated) in asthenozoospermia which by far is the highest number reported earlier. Further, bioinformatics analysis revealed several biological processes, including mitochondrial-related energy production, oxidative phosphorylation (OXPHOS), citric acid cycle (CAC), cytoskeleton, stress response, and protein metabolism altered in asthenozoospermic sperm tail samples. Collectively, our findings reveal the importance of mitochondrial energy production and induced stress response as potential mechanisms involved in the loss of sperm motility in asthenozoospermia.

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All data generated or analysed during this study are included in this published article (and its supplementary information files).

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Acknowledgements

We express our heartfelt appreciation to Morteza Abyadeh for his great help in writing this manuscript. We also thank Mehdi Alikhani for helping with preliminary data analysis and providing valuable contributions.

Funding

This work was supported by a research grant (91000398) from the Royan Institute.

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Authors and Affiliations

  1. Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Shahab Mirshahvaladi

  2. Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia

    Shahab Mirshahvaladi

  3. Cellular and Molecular Research Center, Cellular and Molecular Medicine Research Institute, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

    Tohid Rezaei Topraggaleh

  4. Department of Anatomical Sciences, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

    Tohid Rezaei Topraggaleh

  5. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey

    Mustafa Numan Bucak

  6. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Pegah Rahimizadeh & Abdolhossein Shahverdi

  7. Division of Experimental Surgery, McGill University, Montreal, QC, Canada

    Pegah Rahimizadeh

  8. Cancer Research Program, The Research Institute of the McGill University Health Centre, Montreal, QC, Canada

    Pegah Rahimizadeh

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Contributions

TRT and ASH conceived the idea and designed the study. SM performed mass spectrometry and bioinformatic analysis. TRT, SM, and PR carried out the experiments. The first draft was written by TRT and completed by MNB. All authors read and edited the final version of the manuscript.

Corresponding authors

Correspondence to Tohid Rezaei Topraggaleh or Abdolhossein Shahverdi.

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Ethical approval

This case–control study was approved by the Institutional Ethics Committee of Royan Institute, Tehran, Iran (IR.ACECR.ROYAN.REC.1391.398).

Consent to participate

The written informed consents were signed before semen collection by patients referring to Royan Institute for assisted reproduction treatments. All cases were informed about using their clinical and biological data for this study.

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The authors declare no competing interests.

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Mirshahvaladi, S., Topraggaleh, T.R., Bucak, M.N. et al. Quantitative proteomics of sperm tail in asthenozoospermic patients: exploring the molecular pathways affecting sperm motility. Cell Tissue Res 392, 793–810 (2023). https://doi.org/10.1007/s00441-023-03744-y

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