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The role of copper chromite nanoparticles on physical and bio properties of scaffolds based on poly(glycerol-azelaic acid) for application in tissue engineering fields

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Abstract

Tissue engineering combines suitable cells, engineering methods, and proper biochemical factors to develop functional and biological tissues and repair damaged tissues. In this study, we focused on synthesizing and characterizing a nanocomposite scaffold based on glycerol and azelaic acid (Gl-Az) combined with copper chromite (CuCr2O4) nanoparticles in order to increase the osteogenic differentiation efficiency of human adipose-derived stem cells (hADSCs) on fabricated scaffolds. The degradability and hydrophobicity properties as well as mechanical and thermal behaviors of nanocomposite scaffolds were investigated. Next, the cell toxicity of glycerol, azelaic acid and CuCr2O4 nanoparticles was studied by MTT assay test and acridine orange staining. Finally, the osteogenic differentiation of hADSCs on Gl-Az-CuCr2O4 scaffolds was examined using alkaline phosphatase activity (ALP) and calcium content. The obtained results demonstrated that Gl-Az-1%CuCr2O4 not only showed appropriate mechanical strength, biocompatibility and degradability but also influenced the capability of hADSCs to differentiate into osteogenic lineages. The hADSCs culture in Gl-Az-1%CuCr2O4 showed a significant increase in ALP activity levels and calcium biomineralization after 14 days of osteogenic differentiation. In conclusion, the Gl-Az-1%CuCr2O4 nanocomposite could be used as a biocompatible and degradable scaffold to induce the bone differentiation of hADSCs and it could be a promising scaffold in bone regenerative medicine.

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Acknowledgements

The authors would like to thank the Clinical Research Development Center of Baqiyatallah Hospital and Baqiyatallah University of Medical Sciences for their technical support. The authors also appreciate the useful and helpful comments of Ms. Eftekhari.

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Authors and Affiliations

  1. Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Masoumeh Heydari & Negar Motakef Kazemi

  2. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Vahabodin Goodarzi & Ali Salimi

  3. Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran

    Mehdi Shams

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  1. Masoumeh Heydari
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  2. Vahabodin Goodarzi
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  4. Negar Motakef Kazemi
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  5. Ali Salimi
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Correspondence to Ali Salimi.

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Heydari, M., Goodarzi, V., Shams, M. et al. The role of copper chromite nanoparticles on physical and bio properties of scaffolds based on poly(glycerol-azelaic acid) for application in tissue engineering fields. Cell Tissue Res 391, 357–373 (2023). https://doi.org/10.1007/s00441-022-03708-8

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