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Cell and Tissue Research

, Volume 370, Issue 1, pp 99–112 | Cite as

Cell type-specific localization of Ephs pairing with ephrin-B2 in the rat postnatal pituitary gland

  • Saishu Yoshida
  • Takako Kato
  • Naoko Kanno
  • Naoto Nishimura
  • Hiroto Nishihara
  • Kotaro Horiguchi
  • Yukio KatoEmail author
Regular Article

Abstract

Sox2-expressing stem/progenitor cells in the anterior lobe of the pituitary gland form two types of micro-environments (niches): the marginal cell layer and dense cell clusters in the parenchyma. In relation to the mechanism of regulation of niches, juxtacrine signaling via ephrin and its receptor Eph is known to play important roles in various niches. The ephrin and Eph families are divided into two subclasses to create ephrin/Eph signaling in co-operation with confined partners. Recently, we reported that ephrin-B2 localizes specifically to both pituitary niches. However, the Ephs interacting with ephrin-B2 in these pituitary niches have not yet been identified. Therefore, the present study aims to identify the Ephs interacting with ephrin-B2 and the cells that produce them in the rat pituitary gland. In situ hybridization and immunohistochemistry demonstrated cell type-specific localization of candidate interacting partners for ephrin-B2, including EphA4 in cells located in the posterior lobe, EphB1 in gonadotropes, EphB2 in corticotropes, EphB3 in stem/progenitor cells and EphB4 in endothelial cells in the adult pituitary gland. In particular, double-immunohistochemistry showed cis-interactions between EphB3 and ephrin-B2 in the apical cell membranes of stem/progenitor cell niches throughout life and trans-interactions between EphB2 produced by corticotropes and ephrin-B2 located in the basolateral cell membranes of stem/progenitor cells in the early postnatal pituitary gland. These data indicate that ephrin-B2 plays a role in pituitary stem/progenitor cell niches by selective interaction with EphB3 in cis and EphB2 in trans.

Keywords

Rat pituitary Stem/progenitor cells Niche Ephrin-B2 EphB3 

Abbreviations

CAR

Coxsackievirus and adenovirus receptor

DAPI

4,6-Diamidino-2-phenylindole

Eph

Erythropoietin-producing hepatocellular carcinoma receptor

Ephrin

Eph family receptor interacting proteins

FITC

Fluorescein isothiocyanate

MCL

Marginal cell layer

PCR

Polymerase chain reaction

PDZ

PSD-95, Dlg, ZO-1

PFA

Paraformaldehyde

SOX2

Sex-determining region Y-box 2

SVZ

Subventricular zone

TBP

TATA-box-binding protein

Notes

Acknowledgements

The authors wish to thank Dr. A.F. Parlow and the NIDDK for antibodies against pituitary hormones, Dr. S. Tanaka at Shizuoka University for antibodies against human ACTH and GH and K. Kawai for his excellent technical assistance. This work was partially supported by JSPS KAKENHI (grant numbers 21380184 to YK, 24580435 to TK and 16 K18818 to SY); the MEXT-Supported Program for the Strategic Research Foundation at Private Universities; a research grant (A) to YK from the Institute of Science and Technology, Meiji University; and the Meiji University International Institute for BioResource Research (MUIIR).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Saishu Yoshida
    • 1
    • 2
  • Takako Kato
    • 2
  • Naoko Kanno
    • 1
  • Naoto Nishimura
    • 1
  • Hiroto Nishihara
    • 1
  • Kotaro Horiguchi
    • 3
  • Yukio Kato
    • 1
    • 2
    • 4
    Email author
  1. 1.Division of Life Science, Graduate School of AgricultureMeiji UniversityKanagawaJapan
  2. 2.Institute of Reproduction and EndocrinologyMeiji UniversityKanagawaJapan
  3. 3.Laboratory of Anatomy and Cell Biology, Department of Health SciencesKyorin UniversityTokyoJapan
  4. 4.Department of Life Science, School of AgricultureMeiji UniversityKanagawaJapan

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