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Presence of gonadotropin-releasing hormone-like peptide in the central nervous system and reproductive organs of the male blue swimming crab, Portunus pelagicus, and its effect on spermatogenesis

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Abstract

Our previous studies have demonstrated that lamprey gonadotropin-releasing hormone-III (lGnRH-III)-like peptide occurs in the central nervous system (CNS) of decapod crustaceans (Macrobrachium rosenbergii, Penaeus monodon, Portunus pelagicus), and that lGnRH-III is the most potent in stimulating ovarian maturation compared with other GnRH isoforms. In this study, we examined the localization of lGnRH-III-like peptide in the CNS and male reproductive organs of the blue swimming crab by using anti-lGnRH-III as a probe. In the brain, lGnRH-III immunoreactivity (−ir) was detected in neurons of clusters 6, 10, 11, 14/15, 16, and 17 and in many neuropils. In the subesophageal ganglion, lGnRH-III-ir was present in neurons of the dorso-lateral and ventro-medial clusters. In the thoracic ganglia, lGnRH-III-ir was observed in the large-sized neurons between the thoracic neuropils and in the ventromedial cluster of the abdominal ganglia. In the testis, lGnRH-III-ir was detected in nurse cells, hemocytes, spermatids 2, and the outer and inner zones of the acrosomes of spermatozoa. Bioassay showed that lGnRH-III significantly increased the testis-somatic index, the percentage of late stages of seminiferous tubules (stages VII–IX), the diameter of the seminiferous tubules, and the number of BrdU-labeled early germ cells compared with the control groups. Thus, lGnRH-III-like peptide exists in the male crab and possibly enhances germ cell proliferation and maturation in the testes, leading to increased sperm production.

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Authors and Affiliations

  1. Department of Anatomy, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand

    Thanyaporn Senarai, Wattana Weerachatyanukul, Prasert Sobhon & Prepee Sretarugsa

  2. Department of Anatomy, Faculty of Science, Prince of Songkla University, Songkhla, 90110, Thailand

    Jirawat Saetan

  3. Coastal Aquatic Feed Research Institute, Coastal Fisheries Research and Development Bureau, Department of Fisheries, Petchaburi, 76130, Thailand

    Montakan Tamtin

  4. Faculty of Allied Health Sciences, Burapha University, Chonburi, 20131, Thailand

    Prasert Sobhon

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  1. Thanyaporn Senarai
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  2. Jirawat Saetan
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  3. Montakan Tamtin
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  5. Prasert Sobhon
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Correspondence to Prepee Sretarugsa.

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This research was financially supported by a Distinguished Research Professor Grant (co-funded by Thailand Research Fund, Office of the Higher Education Commission, and Mahidol University) to Prof. Prasert Sobhon, by a grant from the Office of Higher Education, and by Mahidol University under the National Research Universities Initiatives, Thailand Research Fund/Royal Golden Jubilee Ph.D. Program Scholarship to Prof. Prapee Sretarugsa and Thanyaporn Senarai.

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Senarai, T., Saetan, J., Tamtin, M. et al. Presence of gonadotropin-releasing hormone-like peptide in the central nervous system and reproductive organs of the male blue swimming crab, Portunus pelagicus, and its effect on spermatogenesis. Cell Tissue Res 365, 265–277 (2016). https://doi.org/10.1007/s00441-016-2375-0

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