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Gas1 is present in germinal niches of developing dentate gyrus and cortex

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Abstract

Gas1 is a pleiotropic protein that inhibits cell growth when overexpressed in tumors but during development, it acts as a co-receptor for sonic hedgehog to promote the proliferation and survival of various growing organs and systems. This protein has been extensively studied during development in the cerebellum. However, in other structures of the central nervous system, information concerning Gas1 is limited to in situ hybridization studies. We investigate the pattern of Gas1 expression during various developmental stages of the cortex and dentate gyrus of the mouse brain. The levels of Gas1 decrease in the developing brain and the protein is mainly found in progenitor cells during the development of the cortex and dentate gyrus.

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Acknowledgments

We thank Dr. J. Hernández (Cinvestav) for the kind gift of the β-actin antibody and R. Sánchez for technical support.

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Authors and Affiliations

  1. Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN # 2508, México, 07360, D.F., Mexico

    E. Estudillo, P. Zavala, G. Pérez-Sánchez, A. E. Ayala-Sarmiento & J. Segovia

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  1. E. Estudillo
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  2. P. Zavala
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  3. G. Pérez-Sánchez
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  4. A. E. Ayala-Sarmiento
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  5. J. Segovia
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Corresponding author

Correspondence to J. Segovia.

Additional information

This work was partially supported by Conacyt grant 127357 (JS).

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Fig. S1

NIH3T3 cells in serum starved and proliferating conditions. Gas1 (red) is present in high concentrations only in starving NIH3T3 cells. (GIF 43 kb)

High resolution image (TIF 792 kb)

Fig. S2

Immunofluorescence against Gas1 in lung (a) and heart (b) (GIF 139 kb)

High resolution image (TIF 2904 kb)

Fig. S3

Pulse-chase assay during the development of the dentate gyrus: 2 days after BrdU uptake (E16.5). a Panoramic view of a DG of an E16.5 embryo that received BrdU at E14.5. b-e A Gas1/BrdU positive cell at the DMS (arrow) from the inset in a; c-e are magnifications from b. f-i A Gas1/BrdU positive cell at the DG (arrow) from the inset in a; g-i are magnifications from f. DMS dentate migratory stream; DG dentate gyrus. a Scale bar 100 μm; b scale bar 20 μm; c and d scale bars 5 μm; f scale bar 20 μm; g and h scale bar 5 μm. (GIF 491 kb)

High resolution image (TIF 12455 kb)

Fig. S4

Pulse-chase assay during the development of the dentate gyrus: images of a newborn mouse after BrdU uptake at E14.5. a Panoramic view of a DG from a newborn mouse. b-e A Gas1/BrdU positive cell at the dentate gyrus (arrow) along with a BrdU positive cell that lacks of Gas1 (asterisk); c-e are magnifications from b. a Scale bar 100 μm; b scale bar 50 μm; c-d scale bar 10 μm. (GIF 268 kb)

High resolution image (TIF 8100 kb)

Fig. S5

Immunofluorescence of Gas1 and β-tubulin class III in the cortex of an E14.5 embryo. c-d Magnifications from the highest inset in b; e magnification from d; f orthogonal section from e; h orthogonal section from g; i magnification from g; j orthogonal section from i; k magnification from the nearest inset to the LV in b; l orthogonal section from k. LV lateral ventricle; CP cortical plate. a-b Scale bar 100 μm; c-d scale bar 20 μm; scale bar 10 μm in e; scale bar 10 μm in g; scale bar 10 μm in i; scale bar 20 μm in k. (GIF 214 kb)

High resolution image (TIF 9296 kb)

Fig. S6

Immunofluorescence of Gas1 and β-tubulin class III in the cortex of E15.5 embryo. b is a magnification from a; c orthogonal section from b. LV lateral ventricle; CP cortical plate. Scale bar 100 μm in a; scale bar 20 μm in b. (GIF 150 kb)

High resolution image (TIF 4624 kb)

Fig. S7

Immunofluorescence of Gas1 and Gfap expressing cells in the dentate gyrus of newborn mice. b Magnification from the inset in a; c a Gfap/Gas1 positive cell from the inset of b; d orthogonal section from c. Scale bar 100 μm in a; scale bar 20 μm in b; scale bar 5 μm in c. (GIF 333 kb)

High resolution image (TIF 7606 kb)

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Estudillo, E., Zavala, P., Pérez-Sánchez, G. et al. Gas1 is present in germinal niches of developing dentate gyrus and cortex. Cell Tissue Res 364, 369–384 (2016). https://doi.org/10.1007/s00441-015-2338-x

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