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Comparative analysis of neural differentiation potential in human mesenchymal stem cells derived from chorion and adult bone marrow

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Abstract

The finding of a reliable and abundant source of stem cells for the replacement of missing neurons in nervous system diseases requires extensive characterization of neural-differentiation-associated markers in stem cells from various sources. Chorion-derived stem cells from the human placenta have recently been described as an abundant, ethically acceptable, and easily accessible source of cells that are not limited in the same way as bone marrow (BM) mesenchymal stem cells (MSCs). We have isolated and cultured chorion MSCs (C-MSCs) and compared their proliferative capacity, multipotency, and neural differentiation ability with BM-MSCs. C-MSCs showed a higher proliferative capacity compared with BM-MSCs. The expression and histone modification of Nestin, as a marker for neural stem/progenitor cells, was evaluated quantitatively between the two groups. The Nestin expression level in C-MSCs was significantly higher than that in BM-MSCs. Notably, modifications of lys9, lys4, and lys27 of histone H3 agreed with the remarkable higher expression of Nestin in C-MSCs than in BM-MSCs. Furthermore, after neural differentiation of MSCs upon retinoic acid induction, both immunocytochemical and flow cytometry analyses demonstrated that the expression of neural marker genes was significantly higher in neural-induced C-MSCs compared with BM-MSCs. Mature neuron marker genes were also expressed at a significantly higher level in C-MSCs than in BM-MSCs. Thus, C-MSCs have a greater potential than BM-MSCs for differentiation to neural cell lineages and can be regarded as a promising source of stem cells for the cell therapy of neurological disorders.

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Acknowledgments

We thank Raha Favaedi for her kind technical support of the project.

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Authors and Affiliations

  1. Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

    Reihane Ziadlou & Maryam Shahhoseini

  2. Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Reihane Ziadlou, Fatemeh Safari, Forugh-Azam Sayahpour, Shiva Nemati & Mohamadreza Baghaban Eslaminejad

  3. Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran

    Reihane Ziadlou

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  1. Reihane Ziadlou
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  2. Maryam Shahhoseini
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  4. Forugh-Azam Sayahpour
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  5. Shiva Nemati
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  6. Mohamadreza Baghaban Eslaminejad
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Corresponding authors

Correspondence to Maryam Shahhoseini or Mohamadreza Baghaban Eslaminejad.

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The authors dedicate this paper to the memory of Dr. Saeid Kazemi Ashtiani, the late founder of the Royan Institute.

This project was financially supported by grant no. 661 of the Royan Institute.

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Ziadlou, R., Shahhoseini, M., Safari, F. et al. Comparative analysis of neural differentiation potential in human mesenchymal stem cells derived from chorion and adult bone marrow. Cell Tissue Res 362, 367–377 (2015). https://doi.org/10.1007/s00441-015-2210-z

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  • DOI: https://doi.org/10.1007/s00441-015-2210-z

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