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Origin of IgM+IgG+ lymphocytes in the bursa of Fabricius

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Abstract

IgM+IgG+ B cells were detected, by immunofluorescence staining of single cells, in the bursa of Fabricius after hatching. To study the role of maternal IgG (MIgG) in this emergence of IgM+IgG+ B cells, MIgG-free chicks were established from surgically bursectomized hens. Deprivation of MIgG in chicks completely prevented the appearance of IgM+IgG+ B cells in the bursa 1 week after hatching. However, introduction of fluorescein-isothiocyanate-labeled MIgG to MIgG-free chick embryos on day 18 of incubation retrieved IgM+IgG+ B cells in the bursa 1 week after hatching. Thus, IgM+IgG+ B cells are induced by the binding of MIgG to IgM+ B cells in the bursa after hatching. Nevertheless, no binding of MIgG to IgM+ B cells was observed in the bursa of chick embryos in which B-cell proliferation and differentiation were independent of external antigens (Ags). Additionally, the binding of MIgG to IgM+ B cells after hatching was prevented by the isolation of the bursa from environmental stimuli by bursal duct ligation. Therefore, Ag stimulation from the external environment to the bursa is indispensable for the binding of MIgG to IgM+ B cells in the bursa. Taken together, the data demonstrate that IgM+IgG+ B cells are generated by Ag-dependent binding of MIgG to IgM+ B cells in the bursa after hatching.

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Abbreviations

Ab:

Antibody

Ag:

Antigen

Ag-Ab-C:

Antigen-antibody-complement

BCR:

B-cell receptor

BDL:

Bursal duct ligation

CR:

Complement receptor

FAE:

Follicle-associated epithelium

FcγR:

Fcγ receptor

FDC:

Follicular dendritic cell

FITC-MIgG:

Fluorescein isothiocyanate-labeled maternal IgG

GALT:

Gut-associated lymphoid tissue

MBL:

Mannan-binding lectin

MIgG:

Maternal IgG

RMIgG-free:

Restored MIgG-free

SBx-hen:

Surgically bursectomized hen

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Acknowledgments

We thank H. Yokoyama for providing us with MIgG, M. Susa for her critical reading of the manuscript, and S. Kawamura for his encouragement.

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Authors and Affiliations

  1. Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan

    Shigeo Ekino & Kayoko Sonoda

  2. Department of Immunology and Hematology, School of Health Sciences, Kumamoto University, Kuhonji, Kumamoto, Japan

    Seiji Inui

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  1. Shigeo Ekino
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  2. Kayoko Sonoda
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  3. Seiji Inui
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Correspondence to Shigeo Ekino or Seiji Inui.

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This work was supported in part by the Nisshin Seifun Group in Japan.

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Ekino, S., Sonoda, K. & Inui, S. Origin of IgM+IgG+ lymphocytes in the bursa of Fabricius. Cell Tissue Res 362, 153–162 (2015). https://doi.org/10.1007/s00441-015-2196-6

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