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Expression of SGTA correlates with neuronal apoptosis and reactive gliosis after spinal cord injury

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Abstract

Small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) is a novel TPR-containing protein involved in various biological processes. However, the expression and roles of SGTA in the central nervous system remain unknown. We have produced an acute spinal cord injury (SCI) model in adult rats and found that SGTA protein levels first significantly increase, reach a peak at day 3 and then gradually return to normal level at day 14 after SCI. These changes are striking in neurons, astrocytes and microglia. Additionally, colocalization of SGTA/active caspase-3 has been detected in neurons and colocalization of SGTA/proliferating cell nuclear antigen has been detected in astrocytes and microglial. In vitro, SGTA depletion by short interfering RNA inhibits astrocyte proliferation and decreases cyclinA and cyclinD1 protein levels. SGTA knockdown also reduces neuronal apoptosis. We speculate that SGTA is involved in biochemical and physiological responses after SCI.

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Abbreviations

SGTA:

Small glutamine-rich tetratricopeptide repeat-containing protein alpha

SCI:

Spinal cord injury

TPR:

Tetratricopeptide repeat

CNS:

Central nervous system

PCNA:

Proliferating cell nuclear antigen

NeuN:

Neuronal nuclear antigen

GFAP:

Glial fibrillary acidic protein

IBa1:

Ionized calcium-binding adapter molecule 1

siRNA:

Short interfering RNA

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Minhao Chen, Xiaopeng Xia, Jianhua Cao, Yingjie Ni, Yang Liu, Shixian Yan, Xinghai Cheng & Youhua Wang

  2. Department of Orthopaedics, The Second Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Xinhui Zhu & Dawei Xu

  3. Department of Microbiology and Immunology, Medical College, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

    Yonghua Liu

Authors
  1. Minhao Chen
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  2. Xiaopeng Xia
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  3. Xinhui Zhu
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  4. Jianhua Cao
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  5. Dawei Xu
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  6. Yingjie Ni
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  7. Yang Liu
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  8. Shixian Yan
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  9. Xinghai Cheng
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  10. Yonghua Liu
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  11. Youhua Wang
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Corresponding author

Correspondence to Youhua Wang.

Additional information

This work was supported by the National Natural Science Foundation of China (no. 81171140, no. 31300902), the Colleges and Universities in the Natural Science Research Project of Jiangsu Province (13KJB310009) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Chen, M., Xia, X., Zhu, X. et al. Expression of SGTA correlates with neuronal apoptosis and reactive gliosis after spinal cord injury. Cell Tissue Res 358, 277–288 (2014). https://doi.org/10.1007/s00441-014-1946-1

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  • DOI: https://doi.org/10.1007/s00441-014-1946-1

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