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Characterization of a plasma membrane Ca2+ ATPase expressed in olfactory receptor neurons of the moth Spodoptera littoralis

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Abstract

The response of insect olfactory receptor neurons (ORNs) involves an increase in intracellular Ca2+ concentration, as in vertebrate ORNs. In order to decipher the Ca2+ clearance mechanisms in insect ORNs, we have investigated the presence of a plasma membrane Ca2+ ATPase (PMCA) in the peripheral olfactory system of the moth Spodoptera littoralis. From an analysis of a male antennal expressed-sequence-tag database combined with a strategy of 5′/3′ rapid amplification of cDNA ends plus the polymerase chain reaction, we have cloned a full-length cDNA encoding a PMCA. In adult males, the PMCA transcript has been found in various tissues, including the antennae in which its presence has been detected in the sensilla trichodea, and in cultured ORNs. The PMCA gene is slightly expressed at the end of the pupal stage, reaches a maximum at emergence and is maintained at a high level during the adult period. Taken together, these results provide, for the first time, molecular evidence for the putative participation of a PMCA in signalling pathways responsible for the establishment and functioning of the insect peripheral olfactory system.

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Authors and Affiliations

  1. UPMC - Université Paris 6, UMR-A 1272 Physiologie de l’Insecte: Signalisation et Communication, 7 Quai Saint-Bernard, 75252, Paris cedex 05, France

    Adrien François, Françoise Bozzolan, Nicolas Montagné & Stéphane Debernard

  2. INRA, UMR-A 1272 Physiologie de l’Insecte: Signalisation et Communication, Route de Saint-Cyr, 78026, Versailles cedex, France

    Adrien François, Elodie Demondion & Philippe Lucas

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  1. Adrien François
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  2. Françoise Bozzolan
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  4. Nicolas Montagné
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  5. Philippe Lucas
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  6. Stéphane Debernard
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Correspondence to Stéphane Debernard.

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Table S1

Primer sequences. List of primers used for 5′/3′ rapid amplification of cDNA ends (RACE) with the polymerase chain reaction (PCR), reverse transcription with PCR (RT-PCR) and quantitative PCR (qPCR) analyses. (JPEG 57 kb)

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Table S2

Accession numbers of amino acid sequences of plasma membrane Ca2+ ATPase (PMCA)(JPEG 115 kb)

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François, A., Bozzolan, F., Demondion, E. et al. Characterization of a plasma membrane Ca2+ ATPase expressed in olfactory receptor neurons of the moth Spodoptera littoralis . Cell Tissue Res 350, 239–250 (2012). https://doi.org/10.1007/s00441-012-1483-8

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