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BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development

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Abstract

This study evaluated the levels of bone morphogenetic protein receptors BMPRIB and BMPRII mRNA in goat follicles and the effects of bone morphogenetic protein-15 (BMP-15) on the in vitro development of cultured preantral follicles. Real-time polymerase chain reaction (PCR) was used to analyze the levels of BMPRIB and BMPRII mRNA in caprine preantral follicles and in small and large antral follicles. Preantral follicles (≥150 μm) were also isolated from goat ovaries and cultured for 18 days in α-MEM+ supplemented with or without BMP-15 (10, 50, or 100 ng/ml). At the end of culture, some follicles were fixed for ultrastructural evaluation. Real-time PCR showed a reduction in BMPRII mRNA levels from the primary to secondary follicles. Higher levels of BMPRIB mRNA were observed in granulosa/theca cells from large antral follicles compared with small antral follicles. Moreover, BMPRII mRNA was expressed to a greater extent in cumulus–oocyte complexes from large antral follicles than in their respective granulosa/theca cells. In culture, 50 ng/ml BMP-15 positively influenced antral cavity formation and follicle growth after 18 days and also maintained follicular integrity. Thus, BMPRIB and BMPRII mRNAs are present in all follicular categories. BMP-15 (50 ng/ml) stimulates growth, antrum formation and the ultrastructural integrity of isolated caprine preantral follicles after 18 days of culture.

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Acknowledgments

The authors thank Emmanuel Teles Sales for his contribution to the experiments.

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Authors and Affiliations

  1. Faculty of Veterinary, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil

    Isadora Machado T. Lima, Ivina R. Brito, Rafael Rossetto, Ana Beatriz G. Duarte, Giovanna Q. Rodrigues, Márcia Viviane A. Saraiva, José Ricardo de Figueiredo & Ana Paula R. Rodrigues

  2. Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceará, Sobral, CE, Brazil

    José Jackson N. Costa & José Roberto V. Silva

  3. Ultrastructure Laboratory, Aggeu Magalhães Research Center (FIOCRUZ) and Center for Strategic Technologies of the Northeast (CETENE), Recife, PE, Brazil

    Mariana Aragão M. Donato & Christina A. Peixoto

  4. Faculdade de Veterinária – Programa de Pós-Graduação em Ciências Veterinárias (PPGCV) – Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana 1700, Campus do Itaperi, Fortaleza, CE, Brasil

    Isadora Machado T. Lima

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  1. Isadora Machado T. Lima
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  2. Ivina R. Brito
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  3. Rafael Rossetto
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  4. Ana Beatriz G. Duarte
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  5. Giovanna Q. Rodrigues
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  6. Márcia Viviane A. Saraiva
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  7. José Jackson N. Costa
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  8. Mariana Aragão M. Donato
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  9. Christina A. Peixoto
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  10. José Roberto V. Silva
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  11. José Ricardo de Figueiredo
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  12. Ana Paula R. Rodrigues
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Corresponding author

Correspondence to Isadora Machado T. Lima.

Additional information

This work was supported by the National Council for Scientific and Technological Development (CNPq, Brazil, grant number: 554812/2006-1- RENORBIO), the Coordination for the Improvement of Higher Education Personnel (CAPES) and the Brazilian Innovation Agency (FINEP). Isadora Machado Teixeira Lima is a recipient of a grant from CAPES.

The authors declare no potential conflict of interests that can be perceived as prejudicing the impartiality of the research reported.

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Lima, I.M.T., Brito, I.R., Rossetto, R. et al. BMPRIB and BMPRII mRNA expression levels in goat ovarian follicles and the in vitro effects of BMP-15 on preantral follicle development. Cell Tissue Res 348, 225–238 (2012). https://doi.org/10.1007/s00441-012-1361-4

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