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Relative mRNA expression and immunolocalization for transforming growth factor-beta (TGF-β) and their effect on in vitro development of caprine preantral follicles

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Abstract

This study aimed to evaluate the immunolocalization and messenger RNA (mRNA) expression for transforming growth factor-beta (TGF-β) and its receptors (TGF-βRI and RII), as well as mRNA expression for P450 aromatase and FSH receptor in caprine preantral follicles. The effects of TGF-β, FSH alone, or in association on the in vitro follicular development were also assessed. Immunohistochemical analyses showed the expression of TGF-β and its receptors in oocytes of all follicle stages and granulosa cells of primary and secondary follicles. mRNA for TGF-β receptors and for FSH receptor (FSHR) was present in preantral follicles as well as in oocytes and granulosa cells of antral follicles. Isolated secondary follicles were cultured in α-minimum essential medium (MEM) alone or supplemented with either FSH (100 ng/ml), TGF-β (10 ng/ml), or TGF-β + FSH for 18 d. TGF-β increased significantly oocyte diameter when compared to FSH alone and control. After 18 d of culture, all groups showed a significant reduction in P450 aromatase and FSHR mRNA levels in comparison to fresh control. In contrast, treatment with FSH significantly increased the mRNA expression for TGF-β in comparison to fresh control and other treatments. In conclusion, the findings showed that TGF-β and its receptors are present in caprine ovarian follicles. Furthermore, they showed a positive effect on oocyte growth in vitro.

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Acknowledgments

This research was supported by grants from the CNPQ (PPGCV: grant number 870090/2001-1) and FINEP–Brazil. G. Q. Rodrigues is a recipient of a grant from the CNPq (Brazil).

Conflict of interest

The authors declare that there is no potential conflict of interest that can be perceived as prejudicing the impartiality of the research reported.

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Authors and Affiliations

  1. Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil

    G. Q. Rodrigues, I. R. Brito, C. M. G. Silva, A. D. Sales, S. V. Castro, C. H. Lobo, C. C. Campello, A. P. R. Rodrigues & J. R. Figueiredo

  2. Institut Nacional de la Recherche Agronomique (INRA), Physiologie de la Reproduction et des Comportements (PRC), Nouzilly, France

    M. J. Bertoldo, N. Duffard, Y. Locatelli & P. Mermillod

  3. Université François Rabelais, Tours, France

    M. J. Bertoldo, N. Duffard, Y. Locatelli & P. Mermillod

  4. Laboratory of Phisiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil

    V. J. F. Freitas

  5. Laboratório de Manipulação de Oócitos e Folículos Pré-Antrais (LAMOFOPA), Universidade Estadual do Ceará (UECE), Av. Paranjana, 1700, Campus do Itaperi, Fortaleza, CE, 60740-903, Brazil

    G. Q. Rodrigues

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  1. G. Q. Rodrigues
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  2. M. J. Bertoldo
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  3. I. R. Brito
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  4. C. M. G. Silva
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  6. S. V. Castro
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  7. N. Duffard
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  8. Y. Locatelli
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  9. P. Mermillod
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  10. C. H. Lobo
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  11. C. C. Campello
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  12. A. P. R. Rodrigues
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  13. V. J. F. Freitas
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  14. J. R. Figueiredo
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Correspondence to G. Q. Rodrigues.

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Editor: T. Okamoto

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Rodrigues, G.Q., Bertoldo, M.J., Brito, I.R. et al. Relative mRNA expression and immunolocalization for transforming growth factor-beta (TGF-β) and their effect on in vitro development of caprine preantral follicles. In Vitro Cell.Dev.Biol.-Animal 50, 688–699 (2014). https://doi.org/10.1007/s11626-014-9775-9

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