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Cellular distribution of NKCC2 in the gastric mucosa and its response to short-term osmotic shock

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Abstract

The Na+-K+-2Cl- cotransporter-2 (NKCC2) has long been recognized as a “kidney-specific” transporter and is important in salt reabsorption. NKCC2 has been found in the gastric mucosa; however, its cellular distribution and function remain obscure. The present study characterized the distribution pattern of NKCC2 in mammalian gastric mucosa and investigated its response to osmotic challenge. Reverse transcription with the polymerase chain reaction, Western blot and immunofluorescence were used to determine NKCC2 expression and localization. The effect of osmotic shock on NKCC2 expression was studied in isolated gastric mucosa with variable osmolarity treatment. Results from all of the above studies were compared with those of NKCC1. Our data indicated that NKCC1 and NKCC2 were expressed in the gastric mucosa of rat, mouse and human. The mRNA transcripts and proteins for NKCC1 and NKCC2 were broadly expressed in the rat gastric mucosa. In rat and mouse, NKCC1 was largely confined to the lower part of the oxyntic and pyloric gland areas, whereas NKCC2 extended throughout the gastric glands. NKCC1 immunoreactivity was strongly expressed in the parietal and chief cells but was weaker in the mucous cells. NKCC2 was abundantly located in the parietal and mucous cells but faintly distributed in the chief cells. Hypertonic treatment increased the protein level of NKCC1 and caused evident membrane translocation. In contrast, NKCC2 was significantly downregulated and no obvious membrane translocation was observed. Thus, NKCC2 displayed a more ubiquitous distribution in the gastric mucosa and might work coordinately with NKCC1 to maintain cell volume homeostasis under hypertonic conditions.

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Authors and Affiliations

  1. Department of Physiology, School of Basic Medical Sciences, Capital Medical University, No.10 Xitoutiao, You An Men, Beijing, 100069, China

    T. Ji, L. F. Zheng, Q. Wang, Z. F. Dou, Y. Zhang & J. X. Zhu

  2. Department of Biology, College of Science and Health, University of Wisconsin - La Crosse, La Crosse, Wis., USA

    S. Liu

Authors
  1. T. Ji
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  2. S. Liu
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  3. L. F. Zheng
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  4. Q. Wang
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  5. Z. F. Dou
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  6. Y. Zhang
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  7. J. X. Zhu
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Corresponding author

Correspondence to J. X. Zhu.

Additional information

This work was supported by the National Natural Science Foundation of China (30971076, 81170346; to J.X.Z.), Scientific Research Key Program of Beijing Municipal Commission of Education (KZ200910025003; to J.X.Z.), Beijing Natural Science Foundation Program (7121003; to J.X.Z.) and Beijing Municipal Project for Developing Advanced Human Resources for Higher Education (PHR201007110, J.X.Z.).

The authors declare no competing interests.

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Ji, T., Liu, S., Zheng, L.F. et al. Cellular distribution of NKCC2 in the gastric mucosa and its response to short-term osmotic shock. Cell Tissue Res 348, 155–165 (2012). https://doi.org/10.1007/s00441-012-1359-y

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  • DOI: https://doi.org/10.1007/s00441-012-1359-y

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