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The subcommissural organ and the development of the posterior commissure in chick embryos

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Abstract

The subcommissural organ (SCO) is an ependymal differentiation located in the diencephalon under the posterior commissure (PC). SCO-spondin, a glycoprotein released by the SCO, belongs to the thrombospondin superfamily and shares molecular domains with axonal pathfinding molecules. Several lines of evidence suggest a relationship between the SCO and the development of the PC in the chick: (1) their close location to each other, (2) their differentiation at the same developmental stage in the chick, (3) the abnormal PC found in null mutants lacking an SCO and (4) the release by the SCO of SCO-spondin. By application of DiI crystals in the PC of chick embryos, we have identified the neurons that give rise to the PC. Labelling is confined to the magnocellular nucleus of the PC (MNPC). To gain insight into the role of the SCO in PC development, coculture experiments of explants of the MNPC region (MNPCr) from embryos at embryonic day 4 (E4) with SCO explants from E4 or E13 embryos have been performed and the neurite outgrowth from the MNPCr explants has been analysed. In the case of coculture of E4 MNPCr with E4 SCO, the number of neurites growing from the MNPCr is higher at the side facing the SCO. However, when E4 MNPCr and E13 SCO are cocultured, the neurites grow mostly at the side opposite to the SCO. These data suggest that, at early stages of development, the SCO releases some attractive or permissive molecule(s) for the growing of the PC, whereas at later stages, the SCO has a repulsive effect over neurites arising from MNPCr.

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Acknowledgements

The authors are grateful to Dr. de la Rosa (Centro de Investigaciones Biológicas, CSIC, Madrid, Spain) for providing the rabbit polyclonal antibody against Ng-CAM, to José Esteban Casares Mira and Pedro Jiménez Palomo for valuable technical assistance and to David Navas Fernández for his technical assistance in the confocal microscopy study.

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  1. Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071, Málaga, Spain

    Carolina Hoyo-Becerra, Maria Dolores López-Ávalos, Manuel Cifuentes, Rick Visser, Pedro Fernández-Llebrez & Jesus M. Grondona

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  1. Carolina Hoyo-Becerra
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  2. Maria Dolores López-Ávalos
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Correspondence to Jesus M. Grondona.

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C. Hoyo-Becerra and M. D. López-Ávalos contributed equally to this study and should be considered as joint first authors.

C. Hoyo-Becerra was a recipient of a predoctoral fellowship (PFPI) from the Ministerio de Ciencia y Tecnología (Spain). This work was supported by grants from the Ministerio de Ciencia y Tecnología (Spain) (BFU 2006-11754) and the Junta de Andalucía (P07-CVI-03079; SAS-S 0742).

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Hoyo-Becerra, C., López-Ávalos, M.D., Cifuentes, M. et al. The subcommissural organ and the development of the posterior commissure in chick embryos. Cell Tissue Res 339, 383–395 (2010). https://doi.org/10.1007/s00441-009-0899-2

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