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Proliferation and Neuro- and Gliogenesis in Normal and Mechanically Damaged Mesencephalic Tegmentum in Juvenile Chum Salmon, Oncorhynchus keta

  • MECHANISMS OF CELL PROLIFERATION AND DIFFERENTIATION
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Abstract

Processes of proliferation and constitutive neuro- and gliogenesis in the mesencephalic tegmentum of intact juvenile chum salmon, Oncorhynchus keta, and at 3 days after a traumatic injury were studied by immunohistochemistry (IHC) labeling of PCNA, HuCD, and GFAP. In the chum tegmentum, the proliferative activity was revealed both in separate cells and in small cell clusters of the periventricular zone (PVZ). The presence of constitutive neurogenic zones provides the processes of persistent brain growth. After damage to the tegmentum, proliferation in PVZ is activated, the constitutive neurogenic zones reactivate, reactive neurogenic niches form in the parenchyma, and the proliferative activity is also initiated in the centers of secondary proliferation (basal tegmentum). It was first found that traumatic damage to the tegmentum leads to accelerated differentiation of neurons in the subventricular zone (SVZ) and dorsomedial tegmentum as well as to the appearance of HuCD+ cells with the ependymo- and radioglial phenotype in SVZ, which are absent in intact animals. It was first shown that the local foci of posttraumatic neurogenesis, located in the reticular formation parenchyma, and the zones of posttraumatic gliosis, which contribute to a more efficient process of cell migration to the injury area, are formed as a result of tegmentum damage. The data obtained provide new information on the constitutive biology of neural stem cells and their involvement in brain regeneration.

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ACKNOWLEDGMENTS

The work was financially supported by the president of the Russian Federation (grant no. MD-4318.2015.4) and the Far East Program for Basic Research for 2015–2017, Far East Branch, Russian Academy of Sciences (project no. 15-I-6-116, section III).

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Authors and Affiliations

  1. National Scientific Center of Marine Biology, Far East Branch, Russian Academy of Sciences, 690041, Vladivostok, Russia

    E. V. Pushchina & A. A. Varaksin

  2. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, 01024, Kyiv, Ukraine

    E. V. Pushchina

  3. Far Eastern Federal University, 690091, Vladivostok, Russia

    I. A. Kapustyanov

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Abbreviations: BrdU, bromodeoxyuridine; GFAP, glial fibrillary acidic protein; GFAP+, GFAP-immunopositive; GFAP–, GFAP-immunonegative; HuCD, neuronal protein; HuCD+, HuCD-immunopositive; HuCD–, HuCD-immunonegative; PCNA, proliferating cell nuclear antigen; PCNA+, PCNA-immunopositive; PCNA–, PCNA-immunonegative; aNSC, neural stem cell of adult animal; DLT, dorsolateral tegmentum; DMT, dorsomedial tegmentum; DMTN, dorsomedial tegmentum nucleus; ODU, optical density units; CNN, constitutive neurogenic niches; FLL, fasciculus longitudinalis lateralis; MRF, mesencephalic reticular formation; NSC, neural stem cell; OD, optical density; TS, torus semicircularis; PVZ, periventricular zone; RG, radial glia; RNN, reactive neurogenic niches; RF, reticular formation; SVZ, subventricular zone; NI, nucleus isthmi (isthmic nucleus).

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Pushchina, E.V., Kapustyanov, I.A. & Varaksin, A.A. Proliferation and Neuro- and Gliogenesis in Normal and Mechanically Damaged Mesencephalic Tegmentum in Juvenile Chum Salmon, Oncorhynchus keta. Russ J Dev Biol 50, 59–76 (2019). https://doi.org/10.1134/S106236041902005X

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