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Localization of myosin-Va in subpopulations of cells in rat endocrine organs

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Abstract

Myosin-Va is a Ca2+/calmodulin-regulated unconventional myosin involved in the transport of vesicles, membranous organelles, and macromolecular complexes composed of proteins and mRNA. The cellular localization of myosin-Va has been described in great detail in several vertebrate cell types, including neurons, melanocytes, lymphocytes, auditory tissues, and a number of cultured cells. Here, we provide an immunohistochemical view of the tissue distribution of myosin-Va in the major endocrine organs. Myosin-Va is highly expressed in the pineal and pituitary glands and in specific cell populations of other endocrine glands, especially the parafollicular cells of the thyroid, the principal cells of the parathyroid, the islets of Langerhans of the pancreas, the chromaffin cells of the adrenal medulla, and a subpopulation of interstitial testicular cells. Weak to moderate staining has been detected in steroidogenic cells of the adrenal cortex, ovary, and Leydig cells. Myosin-Va has also been localized to non-endocrine cells, such as the germ cells of the seminiferous epithelium and maturing oocytes and in the intercalated ducts of the exocrine pancreas. These data provide the first systematic description of myosin-Va localization in the major endocrine organs of rat.

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Acknowledgments

We are extremely grateful to Ms. Vani Maria Alves Corrêa and Mr. Domingos Soares de Souza Filho for their expertise in tissue sectioning and animal manipulation, respectively, to Mr. Domingos Edmundo Pitta for mastering the technique of polyacrylamide gel production and providing us with the gels, and to Ms. Benedita de Oliveira de Souza and Silvia Regina Andrade for general assistance in our laboratories. We thank the Laboratory of Confocal Microscopy of FMRP-USP, especially the talented microscopists Márcia Sirlene Zardin Graeff and Lenaldo Branco Rocha. We would like to thank Dr. Michela Matteoli and Dr. Michele Solimena for allowing us to use the image shown in Fig. 5a, which they obtained, then in Dr. Pietro De Camilli’s laboratory at Yale University. We also express our gratitude to the Emeritus Professor Dr. Antônio Haddad and Dr. Maria Luisa Paço-Larson at FMRP-USP and to Dr. Marcelo Emílio Beletti at ICBIM-UFU for their excellent teaching of histology and for taking time on many occasions to discuss our histology and immunohistochemistry data with us.

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Authors and Affiliations

  1. Instituto de Genética e Bioquímica, Laboratório de Bioquímica e Biologia Molecular, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil

    Foued S. Espindola, Luciana K. Calabria, Ricardo A. Oliveira, Leandro D. Procópio, Andreia B. P. Lima, Jair P. Cunha-Junior & Milton V. Coelho

  2. Faculdade de Medicina de Ribeirão Preto, Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil

    Silmara R. Banzi, Rodrigo J. Custódio, Ricardo A. Oliveira, Leandro D. Procópio, Iêda M. L. Guedes, Roy E. Larson & Enilza M. Espreafico

  3. Instituto de Biociências, Departamento de Morfologia, Universidade Estadual de São Paulo, Botucatu, São Paulo, Brazil

    Cláudia H. Pellizzon

Authors
  1. Foued S. Espindola
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  2. Silmara R. Banzi
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  3. Luciana K. Calabria
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  4. Rodrigo J. Custódio
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  5. Ricardo A. Oliveira
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  6. Leandro D. Procópio
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  7. Andreia B. P. Lima
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  8. Jair P. Cunha-Junior
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  9. Milton V. Coelho
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  10. Iêda M. L. Guedes
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  11. Cláudia H. Pellizzon
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  12. Roy E. Larson
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  13. Enilza M. Espreafico
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Corresponding authors

Correspondence to Foued S. Espindola or Enilza M. Espreafico.

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We thank the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), the Conselho Nacional de Desenvolvimento Tecnológico (CNPq), and the Fundação de Apoio ao Ensino, Pesquisa e Assistência do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto (FAEPA) for grants to the laboratories of E.M.E. and R.E.L. We are also grateful to CNPq for a scientific initiation fellowship to R.J.C. and a researcher fellowship to E.M.E and R.E.L. The Fundação de Amparo a Pesquisa de Minas Gerais (FAPEMIG) provided grant support to the laboratory of F.S.E. The Coordenadoria de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES) provided doctorate fellowships to I.M.L.G. and master fellowships to L.K.C., A.B.P., and J.P.C.J.

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Espindola, F.S., Banzi, S.R., Calabria, L.K. et al. Localization of myosin-Va in subpopulations of cells in rat endocrine organs. Cell Tissue Res 333, 263–279 (2008). https://doi.org/10.1007/s00441-008-0630-8

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