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The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors

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Abstract

Dopamine receptors have been found in certain populations of non-neuronal cells in the brain, viz., discrete areas of ciliated ependyma and the ependymal cells of the choroid plexus. We have studied the presence of both tyrosine-hydroxylase-immunoreactive nerve fibers and dopamine receptors in the subcommissural organ (SCO), an ependymal brain gland that is located in the roof of the third ventricle and that secretes, into the cerebrospinal fluid, glycoproteins that aggregate to form Reissner’s fiber (RF). Antibodies against D2, D3, D4, and D5 dopamine receptors were used in immunoblots of bovine striatum, fresh SCO, and organ-cultured SCO, and in immunocytochemistry of the bovine, rat, and mouse SCO. Only a few tyrosine-hydroxylase fibers appeared to reach the SCO. However, virtually all the secretory ependymal and hypendymal cells of the SCO immunoreacted with antibodies against D2, D4, and D5 receptors, with the last-mentioned rendering the strongest reaction, especially at the ventricular cell pole of the secretory ependymocytes, suggesting that dopamine might reach the SCO via the cerebrospinal fluid. The antibodies against the four subtypes of receptors revealed corresponding bands in immunoblots of striatum and fresh SCO. Although the cultured SCO displayed dopamine receptors, dopamine had no apparent effect on the expression of the SCO-spondin gene/protein or on the release of RF-glycoproteins (SCO-spondin included) by SCO explants, suggesting that dopamine affects the function(s) of the SCO differently from the secretion of RF-glycoproteins.

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Acknowledgments

The authors are grateful to Dr. A. de la Calle and his collaborators, Dr. Z. Khan, Dr. A. Gutiérrez, and Dr. R. Martín, from the Department of Cell Biology, University of Málaga for the gift of the polyclonal antibodies against dopamine receptors. We also wish to thank to E. Moreira for her help with the immunostaining of frozen sections.

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Authors and Affiliations

  1. Departamento de Biología Celular, Genética y Fisiología, Facultad de Ciencias, Universidad de Málaga, Malaga, Spain

    Mercedes Tomé, Antonio J. Jiménez & Jose Manuel Pérez-Fígares

  2. Instituto de Histología y Patología, Facultad de Medicina, Universidad Austral de Chile, Casilla de Correo, P.O. Box 567, Valdivia, Chile

    Hans Richter, Karin Vio & Esteban M. Rodríguez

  3. Fundación Hospital Carlos Haya, Laboratorio de Investigación Hospital Civil, Malaga, Spain

    F. Javier Bermúdez-Silva

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  1. Mercedes Tomé
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  2. Antonio J. Jiménez
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  3. Hans Richter
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Correspondence to Esteban M. Rodríguez.

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Financial support was provided by grants PI 030756 and Red CIEN, Instituto de Salud Carlos III, Spain (to J.M.P.F.), and 1030265 from Fondecyt, Chile (to E.M.R.)

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Tomé, M., Jiménez, A.J., Richter, H. et al. The subcommissural organ expresses D2, D3, D4, and D5 dopamine receptors. Cell Tissue Res 317, 65–77 (2004). https://doi.org/10.1007/s00441-004-0900-z

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