Abstract
Immunohistochemical techniques were used to study the distribution of serotonin-containing neurons in the nervous system of the slug Limax valentianus. Approximately 350 serotonin-like immunoreactive cell bodies were found in the central nervous system. These were located in the cerebral, pedal, visceral and right parietal ganglia. Most serotonin-like immunoreactive neurons had small cell bodies, which were aggregated into discrete clusters. A pair of previously identified metacerebral giant cells were found on the anterior side of the cerebral ganglion, and two additional pairs of uniquely identifiable, serotonin-like immunoreactive cells were found on the posterior side of the cerebral ganglion. The whole-mount maps of these stained neurons will be useful in further physiological and biochemical studies of olfactory learning at the cellular level in Limax valentianus.
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We thank Drs. Hitoshi Aonuma and Haruhiko Suzuki for technical suggestions and critical discussions.
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This study was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Science, Sports and Technology, Japan (nos. 12307053 and 13771353)
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Shirahata, T., Watanabe, S. & Kirino, Y. Distribution of serotonin-like immunoreactive neurons in the slug Limax valentianus . Cell Tissue Res 315, 285–290 (2004). https://doi.org/10.1007/s00441-003-0820-3
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DOI: https://doi.org/10.1007/s00441-003-0820-3