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Enteric motor neurons form synaptic-like junctions with interstitial cells of Cajal in the canine gastric antrum

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Abstract

Morphological studies have shown synaptic-like structures between enteric nerve terminals and interstitial cells of Cajal (ICC) in mouse and guinea pig gastrointestinal tracts. Functional studies of mice lacking certain classes of ICC have also suggested that ICC mediate enteric motor neurotransmission. We have performed morphological experiments to determine the relationship between enteric nerves and ICC in the canine gastric antrum with the hypothesis that conservation of morphological features may indicate similar functional roles for ICC in mice and thicker-walled gastrointestinal organs of larger mammals. Four classes of ICC were identified based on anatomical location within the tunica muscularis. ICC in the myenteric plexus region (IC-MY) formed a network of cells that were interconnected to each other and to smooth muscle cells by gap junctions. Intramuscular interstitial cells (IC-IM) were found in muscle bundles of the circular and longitudinal layers. ICC were located along septa (IC-SEP) that separated the circular muscle into bundles and were also located along the submucosal surface of the circular muscle layer (IC-SM). Immunohistochemistry revealed close physical associations between excitatory and inhibitory nerve fibers and ICC. These contacts were synaptic-like with pre- and postjunctional electron-dense regions. Synaptic-like contacts between enteric neurons and smooth muscle cells were never observed. Innervated ICC formed gap junctions with neighboring smooth muscle cells. These data show that ICC in the canine stomach are innervated by enteric neurons and express similar structural features to innervated ICC in the murine GI tract. This morphology implies similar functional roles for ICC in this species.

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Acknowledgement

The authors are extremely grateful to Julia R. Bayguinov for excellent technical assistance with the immunohistochemical studies.

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Authors and Affiliations

  1. Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV 89557, USA

    Kazuhide Horiguchi, Kenton M. Sanders & Sean M. Ward

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  1. Kazuhide Horiguchi
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  2. Kenton M. Sanders
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  3. Sean M. Ward
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Correspondence to Sean M. Ward.

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This work was supported by a research grant from the National Institute of Diabetes & Digestive & Kidney of the National Institutes of Health, DK57236. Facilities within a Morphology Core Laboratory supported by Program Project Grant DK41315 were used for the immunohistochemical and electron-microscopy studies.

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Horiguchi, K., Sanders, K.M. & Ward, S.M. Enteric motor neurons form synaptic-like junctions with interstitial cells of Cajal in the canine gastric antrum. Cell Tissue Res 311, 299–313 (2003). https://doi.org/10.1007/s00441-002-0657-1

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