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Cloning of caf1 +, caf2  + and caf4  + from Schizosaccharomyces pombe : their involvement in multidrug resistance, UV and pH sensitivity

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Abstract

We previously identified four nuclear genes (caf1 + to caf4 +) in Schizosaccharomyces pombe, mutations in which can confer caffeine resistance. Here we report the cloning and sequencing of caf1 +, caf2 + and caf4 +. All three genes are allelic to genes (hba1 +, crm1 + and trr1 +, respectively) involved in multidrug resistance mechanisms or in stress response systems. In agreement with this the caffeine-resistant mutants caf1(hba1)-21, caf2(crm1)-3 and caf4(trr1)-83 are also resistant to brefeldin. Disruption of caf1(hba1) + and caf4(trr1) + makes cells sensitive to high pH. The overlapping ranges of pleiotropic effects and the genetic interaction detected between caf1(hba1) + and caf2(crm1) + suggest that the three genes function in interlinked systems.

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Authors and Affiliations

  1. Trafford Centre, University of Sussex, Falmer Brighton, BN1 9RY, UK e-mail: peterdora@tigris.klte.hu Fax: +36-52-348550, , , , , , GB

    Z. Benko

  2. Department of Genetics, University of Debrecen PO Box 56, H-4010 Debrecen, Hungary, , , , , , HU

    M. Sipiczki

  3. MRC Cell Mutation Unit, University of Sussex Falmer, Brighton, BN1 9RR, UK, , , , , , GB

    A. M. Carr

Authors
  1. Z. Benko
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  2. M. Sipiczki
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  3. A. M. Carr
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Received: 9 March 1998 / Accepted: 16 September 1998

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Benko, Z., Sipiczki, M. & Carr, A. Cloning of caf1 +, caf2  + and caf4  + from Schizosaccharomyces pombe : their involvement in multidrug resistance, UV and pH sensitivity. Mol Gen Genet 260, 434–443 (1998). https://doi.org/10.1007/s004380050914

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  • DOI: https://doi.org/10.1007/s004380050914

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