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Pps1, phosphatidylserine synthase, regulates the salt stress response in Schizosaccharomyces pombe

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Abstract

Phosphatidylserine (PS) is important for maintaining growth, cytoskeleton, and various functions in yeast; however, its role in stress responses is poorly understood. In Schizosaccharomyces pombe, the PS synthase deletion (pps1∆) mutant shows defects in growth, morphology, cytokinesis, actin cytoskeleton, and cell wall integrity, and these phenotypes are rescued by ethanolamine supplementation. Here, we evaluated the role of Pps1 in the salt stress response in S. pombe. We found that pps1∆ cells are sensitive to salt stresses such as KCl and CaCl2 even in the presence of ethanolamine. Loss of the functional cAMP-dependent protein kinase (git3∆ or pka1∆) or phospholipase B Plb1 (plb1∆) enhanced the salt stress-sensitive phenotype in pps1∆ cells. Green fluorescent protein (GFP)-Pps1 was localized at the plasma membrane and endoplasmic reticulum regardless of the stress conditions. In pka1∆ cells, GFP-Pps1 was accumulated around the nucleus under the KCl stress. Pka1 was localized in the nucleus and the cytoplasm under normal conditions and transferred from the nucleus to the cytoplasm under salt-stress conditions. Pka1 translocated from the nucleus to the cytoplasm during CaCl2 stress in the wild-type cells, while it remained localized in the nucleus in pps1∆ cells. Expression and phosphorylation of Pka1-GFP were not changed in pps1∆ cells. Our results demonstrate that Pps1 plays an important role in the salt stress response in S. pombe.

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The data presented in this study are available in the article, and the data are available from the corresponding author upon request.

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Acknowledgements

The authors thank all the members of our laboratory for their support and scientific advice. We would like to thank Editage (www.editage.com) for English language editing.

Funding

This work was supported by JSPS KAKENHI Grant Numbers JP18K05438 (to YM) and JP19K222831 (to MK).

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GN performed the experiments; MK analyzed the data and provided advice; YM planned the study, designed the experiments, made the yeast strains, performed the experiments, and analyzed the data. YM drafted the original manuscript. MK and YM reviewed and edited the original manuscript.

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Correspondence to Yasuhiro Matsuo.

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Communicated by Andres Aguilera.

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Naozuka, G., Kawamukai, M. & Matsuo, Y. Pps1, phosphatidylserine synthase, regulates the salt stress response in Schizosaccharomyces pombe. Mol Genet Genomics 299, 43 (2024). https://doi.org/10.1007/s00438-024-02135-4

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  • DOI: https://doi.org/10.1007/s00438-024-02135-4

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