Abstract
To understand the lifespan of higher organisms, including humans, it is important to understand lifespan at the cellular level as a prerequisite. So, fission yeast is a good model organism for the study of lifespan. To identify the novel factors involved in longevity, we are conducting a large-scale screening of long-lived mutant strains that extend chronological lifespan (cell survival in the stationary phase) using fission yeast. One of the newly acquired long-lived mutant strains (No.98 mutant) was selected for analysis and found that the long-lived phenotype was due to a missense mutation (92Phe → Ile) in the plb1+ gene. plb1+ gene in fission yeast is a nonessential gene encoding a homolog of phospholipase B, but its functions under normal growth conditions, as well as phospholipase B activity, remain unresolved. Our analysis of the No.98 mutant revealed that the plb1 mutation reduces the integrity of the cellular membrane and cell wall and activates Sty1 via phosphorylation.
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Acknowledgements
The authors thank the National Bio-Resource Project (NBRP) (YGRC) of the MEXT, Japan for some strains. This work was supported by the Japan Society for the Promotion of Science KAKENHI Grant Numbers JP17H03792, JP20H02898, and JP23H02125 (to H.A.).
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Conceptualization, HA; investigation, YM, and KM; writing–original draft, YM, and HA; review and editing, TS, HO, MT, KI, and HA. All authors have read and agreed to the published version of the manuscript.
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438_2024_2107_MOESM1_ESM.pptx
Supplementary file1 Supplementary Figure 1 (a) The chromosome structure of the constructed plb1-98:hygR mutant was shown schematically with the distances from plb1 to hygR cassette and hygR cassette to SPAC1A6.11+. (b) plb1+-mRNA level in WT, No.98, ∆plb1, plb1-hygR, and plb1-98-hygR strains were measured by real-time PCR analysis (n = 3). (c) SPAC1A6.11-mRNA level in WT, No.98, Δplb1, plb1-hygR, and plb1-98-hygR strains were measured by real-time PCR analysis (n = 3) (PPTX 75 KB)
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Maekawa, Y., Matsui, K., Okamoto, K. et al. Identification of plb1 mutation that extends longevity via activating Sty1 MAPK in Schizosaccharomyces pombe. Mol Genet Genomics 299, 20 (2024). https://doi.org/10.1007/s00438-024-02107-8
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DOI: https://doi.org/10.1007/s00438-024-02107-8