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Genome-wide identification of the key kinesin genes during fiber and boll development in upland cotton (Gossypium hirsutum L.)

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Abstract

Kinesin is a kind of motor protein, which interacts with microtubule filaments and regulates cellulose synthesis. Cotton fiber is a natural model for studying the cellular development and cellulose synthesis. Therefore, a systematic research of kinesin gene family in cotton (Gossypium spp.) will be beneficial for both understanding the function of kinesin protein and assisting the fiber improvement. Here, we aimed to identify the key kinesin genes present in cotton by combining genome-wide expression profile data, association mapping, and public quantitative trait loci (QTLs) in upland cotton (G. hirsutum L.). Results showed that 159 kinesin genes, including 15 genes of the kinesin-13 gene subfamily, were identified in upland cotton; of which 157 kinesin genes can be traced back to the diploid ancestors, G. raimondii and G. arboreum. Using a combined analysis of public QTLs and genome-wide expression profile information, there were 29 QTLs co-localized together with 28 kinesin genes in upland cotton, including 10 kinesin-13 subfamily genes. Genome-wide expression profile data indicated that, among the 28 co-localized genes, seven kinesin genes were predominantly expressed in fibers or ovules. By association mapping analysis, 30 kinesin genes were significantly associated with three fiber traits, among which a kinesin-13 gene, Ghir_A11G028430, was found to be associated with both cotton boll length and lint weight, and one kinesin-7 gene, Ghir_D04G017880 (Gh_Kinesin7), was significantly associated with fiber strength. In addition, two missense mutations were identified in the motor domain of the Gh_Kinesin7 protein. Overall, the kinesin gene family seemingly plays an important role in cotton fiber and boll development. The exploited kinesin genes will be beneficial for the genetic improvement of fiber quality and yield.

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The data presented in this study are available in supplementary file.

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Funding

This project was supported by the Science and Technology Research Project of Jiangxi Province, China (Grant No. 20212BAB215009), and the Science and Technology Research Project of the Education Department of Jiangxi Province, China (Grant No. GJJ200440).

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Authors and Affiliations

  1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, Jiangxi, China

    Hong Zhu, Jianfei Wu, Huifang Xu, Shubin Wang & Tianwang Wen

  2. Stock seed farm of Gao’an, Yichun, 330800, Jiangxi, China

    Jianzhong Xu

  3. Xishuangbanna Institute of Agricultural Science, Xishuangbanna Autonomous Prefecture, Yunnan, 666100, China

    Kanbing Yu

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  1. Hong Zhu
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Contributions

TW: designed this project and analyzed the data, HZ and TW: drafted the manuscript, SW and HX: revised the manuscript, JX, KY, HZ and JW: collected the field data.

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Correspondence to Tianwang Wen.

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Zhu, H., Xu, J., Yu, K. et al. Genome-wide identification of the key kinesin genes during fiber and boll development in upland cotton (Gossypium hirsutum L.). Mol Genet Genomics 299, 38 (2024). https://doi.org/10.1007/s00438-024-02093-x

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