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Transcriptome and metabolome profiling in different stages of infestation of Eucalyptus urophylla clones by Ralstonia solanacearum

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Abstract

Eucalyptus urophylla is an economically important tree species that widely planted in tropical and sub-tropical areas around the world, which suffers significant losses due to Ralstonia solanacearum. However, little is known about the molecular mechanism of pathogen-response of Eucalyptus. We collected the vascular tissues of a E. urophylla clone infected by R. solanacearum in the laboratory, and combined transcriptome and metabolome analysis to investigate the defense responses of Eucalyptus. A total of 11 flavonoids that differentially accumulated at the first stage or a later stage after infection. The phenylpropanoid of p-coumaraldehyde, the two alkaloids trigonelline and dl-ephedrine, two types of traditional Chinese medicine with patchouli alcohol and 3-dihydrocadambine, and the amino acid phenylalanine were differentially accumulated after infection, which could be biomarkers indicating a response to R. solanacearum. Differentially expressed genes involved in plant hormone signal transduction, phenylpropanoids, flavonoids, mitogen-activated protein kinase (MAPK) signaling, and amino acid metabolism were activated at the first stage of infection or a later stage, indicating that they may participate in the defense against infection. This study is expected to deliver several insights into the molecular mechanism in response to pathogens in E. urophylla, and the findings have far-reaching implications in the control of E. urophylla pathogens.

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Availability of data and materials

The raw sequence data reported in this paper have been deposited in the Genome Sequence Archive (Genomics, Proteomics & Bioinformatics 2021) in National Genomics Data Center (Nucleic Acids Res 2021), China National Center for Bioinformation/Beijing Institute of Genomics, Chinese Academy of Sciences, under accession number CRA005055 that are publicly accessible at https://ngdc.cncb.ac.cn/gsa. The materials obtained and used in this study are available from the corresponding author on reasonable request.

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Acknowledgements

The research is supported by “the Fundamental Research Funds for the Central Universities” and National Natural Science Foundation of China (31700590). The supporters did not play any role in the design of the study, data collection, and analysis of the data or writing the manuscript.

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Author notes

  1. Yang Xiaohui, Huang Jie and Yang Huixiao contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, No. 233, Guangshan First Road, Guangzhou, 510520, Guangdong, People’s Republic of China

    Yang Xiaohui, Huang Jie, Yang Huixiao, Liao Huanqin, Xu Fang, Zhu Baozhu, Xu Xiuyu, Huang Huayi & Pan Wen

  2. Guangdong Academy of Forestry, No. 233, Guangshan First Road, Guangzhou, 510520, Guangdong, People’s Republic of China

    Yang Xiaohui, Huang Jie, Yang Huixiao, Liao Huanqin, Xu Fang, Zhu Baozhu, Xu Xiuyu, Huang Huayi & Pan Wen

  3. Dongmen State Forestry Farm of Guangxi Zhuang, No. 10, Jinlong Road, Fusui, 532108, Guangxi, People’s Republic of China

    Zhang Lei

  4. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China

    Du Qingzhang

  5. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China

    Du Qingzhang

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  1. Yang Xiaohui
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Contributions

WP and DQZ conceived and designed the experiments; XHY, HJ, HXY, HQL and FX performed the experiments; XXY instructed the experiments; ZL sampled the R. solanacearum; HHY purified the R. solanacearum; XHY and HXY analyzed the data; XHY wrote the manuscript. All authors have read and approved the manuscript.

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Correspondence to Du Qingzhang or Pan Wen.

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Xiaohui, Y., Jie, H., Huixiao, Y. et al. Transcriptome and metabolome profiling in different stages of infestation of Eucalyptus urophylla clones by Ralstonia solanacearum. Mol Genet Genomics 297, 1081–1100 (2022). https://doi.org/10.1007/s00438-022-01903-4

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