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terMITEs: miniature inverted-repeat transposable elements (MITEs) in the termite genome (Blattodea: Termitoidae)

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Abstract

Transposable elements (TEs) are discrete DNA sequences which are able to replicate and jump into different genomic locations. Miniature inverted-repeats TEs (MITEs) are non-autonomous DNA elements whose origin is still poorly understood. Recently, some MITEs were found to contain core repeats that can be arranged in tandem arrays; in some instances, these arrays have even given rise to satellite DNAs in the (peri)centromeric region of the host chromosomes. I report the discovery and analysis of three new MITEs found in the genome of several termite species (hence the name terMITEs) in two different families. For two of the MITEs (terMITE1—Tc1/mariner superfamily; terMITE2—piggyBac superfamily), evidence of past mobility was retrieved. Moreover, these two MITEs contained core repeats, 16 bp and 114 bp long respectively, exhibiting copy number variation. In terMITE2, the tandem duplication appeared associated with element degeneration, in line with a recently proposed evolutionary model on MITEs and the origin of tandem arrays. Concerning their genomic distribution, terMITE1 and terMITE3 appeared more frequently inserted close to coding regions while terMITE2 was mostly associated with TEs. Although MITEs are commonly distributed in coding regions, terMITE2 distribution is in line with that of other insects’ piggyBac-related elements and of other small TEs found in termite genomes. This has been explained through insertional preference rather than through selective processes. Data presented here add to the knowledge on the poorly exploited polyneopteran genomes and will provide an interesting framework in which to study TEs’ evolution and host’s life history traits.

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Acknowledgments

The present work was supported by RFO-University of Bologna and Canziani funding. I wish to thank Barbara Mantovani and Miroslav Plohl for their suggestions and fruitful discussions. I am grateful to Danna G. Eickbush for the critical reading and the language editing of the manuscript. I wish also to thank two anonymous Reviewers, whose criticisms and suggestions helped to substantially improve the paper.

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  1. Dipartimento di Scienze Biologiche, Geologiche e Ambientali, Università di Bologna, via Selmi 3, 40126, Bologna, Italy

    Andrea Luchetti

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Correspondence to Andrea Luchetti.

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Communicated by S. Hohmann.

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Luchetti, A. terMITEs: miniature inverted-repeat transposable elements (MITEs) in the termite genome (Blattodea: Termitoidae). Mol Genet Genomics 290, 1499–1509 (2015). https://doi.org/10.1007/s00438-015-1010-1

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