Abstract
The Ecl1 family genes extend the lifespan of fission yeast when overexpressed. They also cause resistance against H2O2 stress. In this study, we found that the bZip transcription factor Atf1 is a direct activator of the induction of extender of chronological lifespan (ecl1 +) by H2O2 stress. Based on ChIP analysis, we identified that Atf1 binds to the upstream DNA region of ecl1 +. Previously, we reported that overexpression of ecl1 + increased the expression of the catalase-encoding ctt1 +. This ecl1 +-dependent increase of ctt1 + expression occurred in ∆atf1 mutant. On the other hand, the activation of ctt1 + caused by the ∆pyp1 mutation, which enhances Sty1–Atf1 activity, could occur in ∆ecl1 mutant. Based on these results, we propose that Atf1 can regulate ctt1 + in both an Ecl1-dependent and an Ecl1-independent manner.
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Acknowledgments
We thank Dr. K. Azuma (our laboratory) for helpful discussions and Dr. K. Shiozaki (Nara Institute of Science and Technology, Japan) and Dr. M. Yamamoto (The University of Tokyo, Japan) for strains. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan [to HA]. Part of this work was supported by A Research for Promoting Technological Seeds from JST, Nagase Science and Technology Foundation, and The Asahi Glass Foundation [to HA].
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Communicated by S. Hohmann.
T. Shimasaki and H. Ohtsuka contributed equally to this work.
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Shimasaki, T., Ohtsuka, H., Naito, C. et al. Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe . Mol Genet Genomics 289, 685–693 (2014). https://doi.org/10.1007/s00438-014-0845-1
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DOI: https://doi.org/10.1007/s00438-014-0845-1