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Screening for long-lived genes identifies Oga1, a guanine-quadruplex associated protein that affects the chronological lifespan of the fission yeast Schizosaccharomyces pombe

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Abstract

Schizosaccharomyces pombe and Saccharomyces cerevisiae are excellent model organisms to study lifespan. We conducted screening to identify novel genes that, when overexpressed, extended the chronological lifespan of fission yeast. We identified seven genes, among which we focused on SPBC16A3.08c. The gene product showed similarity to Ylr150w of S. cerevisiae, which has affinity for guanine-quadruplex nucleic acids (G4). The SPBC16A3.08c product associated with G4 in vitro and complemented the phenotype of an S. cerevisiae Ylr150w deletion mutant. From these results, we proposed that SPBC16A3.08c encoded for a functional homolog of Ylr150w, which we designated ortholog of G4-associated protein (oga1 +). oga1 + overexpression extended the chronological lifespan and also decreased mating efficiency and caused both high and low temperature-sensitive growth. Deleting oga1 + resulted in caffeine-sensitive and canavanine-resistant phenotypes. Based on these results, we discuss the function of Oga1 on the chronological lifespan of fission yeast.

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Acknowledgments

We thank K. Azuma for helpful discussions, M. Yamamoto (The University of Tokyo, Japan) and The National BioResource Project/Yeast Genetic Resource Center for yeast strains, and T. Ushimaru (Shizuoka University) for plasmids. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This work was also partly supported by A Research for Promoting Technological Seeds from JST, Nagase Science and Technology Foundation, and The Asahi Glass Foundation.

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Authors and Affiliations

  1. Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan

    Hokuto Ohtsuka, Erika Sakai, Keiko Ichinose & Hirofumi Aiba

  2. Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan

    Shingo Ogawa & Hideaki Kawamura

  3. Department of Biological Science, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-ku, Tokyo, 112-8551, Japan

    Hiroshi Murakami

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  1. Hokuto Ohtsuka
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  2. Shingo Ogawa
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  3. Hideaki Kawamura
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  4. Erika Sakai
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  5. Keiko Ichinose
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  6. Hiroshi Murakami
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  7. Hirofumi Aiba
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Correspondence to Hirofumi Aiba.

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Communicated by M. Collart.

H. Ohtsuka and S. Ogawa contributed equally to this work.

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438_2013_748_MOESM1_ESM.pdf

Fig. S1. Seven plasmids that remarkably extend the chronological lifespan of fission yeast. (A) Plasmids that conferred long chronological lifespans to cells included: No. 8 (closed circles), No. 10 (closed triangles), No. 16 (open triangles), No. 148 (closed squares), No. 216 (open squares), No. 226 (closed diamonds), and No. 241 (open diamonds). As a control, the lifespan of cells harboring a vector plasmid, pLB-Dblet (open circles), was determined at the same time. Strain survival rates were determined at least 3 times and essentially same results were obtained. One representative data were shown here. (B) Insert fragments of each plasmid were schematically shown. The numbers on each fragment correspond to the nucleotide positions in the chromosome shown in parenthesis. The genes that caused lifespan extension are shown in boldface. To identify genes that affected lifespan, restriction analyses were performed for some DNA fragments. Each closed triangle indicates the cut site along with the restriction enzyme name (PDF 136 kb)

438_2013_748_MOESM2_ESM.pdf

Fig. S2. Alignment of Stm1 (Ylr150w) of S. cerevisiae and Oga1 (SPBC16A3.08c) of S. pombe. Alignments of the amino acids sequences for Stm1 (Ylr150w) of S. cerevisiae and Oga1 (SPBC16A3.08c) of S. pombe. Identical amino acids between both sequences are linked by lines. Similar amino acids are marked by dots (PDF 99 kb)

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Ohtsuka, H., Ogawa, S., Kawamura, H. et al. Screening for long-lived genes identifies Oga1, a guanine-quadruplex associated protein that affects the chronological lifespan of the fission yeast Schizosaccharomyces pombe . Mol Genet Genomics 288, 285–295 (2013). https://doi.org/10.1007/s00438-013-0748-6

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