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A major QTL located on chromosome V associates with in vitro tuberization in a tetraploid potato population

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Abstract

The cultivated potato (Solanum tuberosum L.) is an autotetraploid species. The complexity of tetrasomic inheritance and the lack of pure lines increase the difficulty of genetic analysis of the inherited characteristics. Tuberization is the determinant step for economic yield of potato. To understand the complex genetic basis of tuberization of the cultivated potato, we developed linkage maps for a tetraploid population (F1) of 237 genotypes and mapped QTLs for the percent of in vitro tuberized plantlets (% IVT). The paternal map for E108 (well tuberized) covered 948 cM and included 12 linkage groups, all of which contained all four homologous chromosomes. The maternal map for E20 (nontuberized) covered 1,286 cM and included 14 linkage groups, 12 of which contained all four homologous chromosomes. All 12 chromosomes of potato were tagged using the SSR markers. A major QTL (MT05) with additive effect was detected on chromosome V of E108 which explained 16.23 % of the variation for % IVT, and two minor QTLs (mt05 and mt09) displaying simplex dominant effects were located on chromosome V and chromosome IX of E20 which explained 5.33 and 4.59 % of the variation for % IVT, respectively. Based on the additive model of MT05, the segregation ratio of the gametic genotypes (Q−: qq = 5:1) matched the ratio of the tuberized genotypes to the nontuberized genotypes in the population suggesting that the segregation of in vitro tuberization in this population is controlled by a major-effect gene or genes. The mapping results of three important candidate genes indicated that the QTL causal genes detected in our study are new. In this study, we developed the almost complete linkage maps of a tetraploid population, identified a major QTL on chromosome V affecting in vitro tuberization, suggested a major-effect gene with minor modifiers model controlling this trait and found that the QTLs identified here correspond to new tuberization genes. Our work provides new and useful information about the genetic basis for tuberization of this autotetraploid crop.

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Acknowledgments

This work was supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-10-P08). We are thankful to Dr. Yongbing Ou and Dr. Jingcai Li for reading this manuscript and giving kind advice. The experiments comply with the current laws of China where they were performed.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. National Center for Vegetable Improvement (Central China), Wuhan, China

    Jun Zhou, Hui Fang, Jianwei Shan, Xiaoxi Gao, Lin Chen, Conghua Xie, Tingting Xie & Jun Liu

  2. Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, Wuhan, China

    Jun Zhou, Hui Fang, Jianwei Shan, Xiaoxi Gao, Lin Chen, Conghua Xie, Tingting Xie & Jun Liu

  3. Potato Engineering and Technology Research Center of Hubei Province, Huazhong, China

    Jun Zhou, Hui Fang, Jianwei Shan, Xiaoxi Gao, Lin Chen, Conghua Xie, Tingting Xie & Jun Liu

  4. Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Jun Zhou, Hui Fang, Jianwei Shan, Xiaoxi Gao, Lin Chen, Conghua Xie, Tingting Xie & Jun Liu

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  1. Jun Zhou
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Correspondence to Jun Liu.

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Zhou, J., Fang, H., Shan, J. et al. A major QTL located on chromosome V associates with in vitro tuberization in a tetraploid potato population. Mol Genet Genomics 289, 575–587 (2014). https://doi.org/10.1007/s00438-014-0832-6

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