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Trans-specific S-RNase and SFB alleles in Prunus self-incompatibility haplotypes

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Abstract

Self-incompatibility in the genus Prunus is controlled by two genes at the S-locus, S-RNase and SFB. Both genes exhibit the high polymorphism and high sequence diversity characteristic of plant self-incompatibility systems. Deduced polypeptide sequences of three myrobalan and three domestic plum S-RNases showed over 97% identity with S-RNases from other Prunus species, including almond, sweet cherry, Japanese apricot and Japanese plum. The second intron, which is generally highly polymorphic between alleles was also remarkably well conserved within these S-allele pairs. Degenerate consensus primers were developed and used to amplify and sequence the co-adapted polymorphic SFB alleles. Sequence comparisons also indicated high degrees of polypeptide sequence identity between three myrobalan and the three domestic plum SFB alleles and the corresponding Prunus SFB alleles. We discuss these trans-specific allele identities in terms of S-allele function, evolution of new allele specificities and Prunus taxonomy and speciation.

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Acknowledgments

We thank Dr Encarna Ortega at CEBAS-CSIC (Spain) and Dr Simon Vaughan (EMR) for providing SFB allele sequence data for almond and sweet cherry. Thanks also to Emma-Jane Lamont at the National Fruit Collections at Brogdale (UK), Dr Encarna Ortega (CEBAS-CSIC) and Dr Yoshihiko Sato at the National Institute of Fruit Tree Science (Japan) for supplying Prunus material, and to Javier-Maria de Vera y Asensio for help with preparing the graphics. Bruce Sutherland gratefully acknowledges a PhD studentship from the University of Nottingham and the East Malling Trust for Horticultural Research.

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Authors and Affiliations

  1. East Malling Research, New Road, East Malling, Kent, ME19 6BJ, UK

    Bruce G. Sutherland & Kenneth R. Tobutt

  2. Plant Science Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK

    Bruce G. Sutherland & Timothy P. Robbins

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  1. Bruce G. Sutherland
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  2. Kenneth R. Tobutt
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  3. Timothy P. Robbins
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Correspondence to Bruce G. Sutherland.

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Communicated by R. Herrmann.

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Sutherland, B.G., Tobutt, K.R. & Robbins, T.P. Trans-specific S-RNase and SFB alleles in Prunus self-incompatibility haplotypes. Mol Genet Genomics 279, 95–106 (2008). https://doi.org/10.1007/s00438-007-0300-7

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