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The phospholipase B homolog Plb1 is a mediator of osmotic stress response and of nutrient-dependent repression of sexual differentiation in the fission yeast Schizosaccharomyces pombe

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Abstract

Although phospholipase B (PLB) enzymes have been described in eukaryotes from yeasts to mammals, their biological functions are poorly understood. Here we describe the characterization of plb1, one of five genes predicted to encode PLB homologs in the fission yeast, Schizosaccharomyces pombe. The plb1 gene is dispensable under normal growth conditions but required for viability in high-osmolarity media and for normal osmotic stress-induced gene expression. Unlike mutants defective in function for the stress-activated MAP kinase Spc1, plb1Δ cells are not hypersensitive to oxidative or temperature stresses, nor do they undergo a G2-specific arrest in response to osmotic stress. In addition to defects in osmotic stress response, plb1Δ cells exhibit a cold-sensitive defect in nutrient-mediated mating repression, a phenotype reminiscent of mutants in the cyclic AMP (cAMP) pathway. We show that, like plb1Δ cells, mutants in the cAMP pathway are defective for growth in high-osmolarity media, demonstrating a previously unrecognized role for the cAMP pathway in osmotic stress response. Furthermore, we show that gain-of function in the cAMP pathway can rescue the osmosensitive growth defect of plb1Δ cells, suggesting that the cAMP pathway is a potential downstream target of the actions of Plb1 in S. pombe.

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Acknowledgements

This work was supported by research grants GM46226 from the National Institutes of Health (C.S.H.) and SR99-263 from the Pharmacia Corporation (S.M.)

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Authors and Affiliations

  1. Department of Molecular Genetics and Program in Genes and Development, M.D. Anderson Cancer Center , University of Texas, 1515 Holcombe Blvd., Houston, TX 77030, USA

    P. Yang, H. Du & S. Marcus

  2. Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA

    C. S. Hoffman

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  1. P. Yang
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  2. H. Du
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  3. C. S. Hoffman
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  4. S. Marcus
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Correspondence to S. Marcus.

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Communicated by E. Di Mauro

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Yang, P., Du, H., Hoffman, C.S. et al. The phospholipase B homolog Plb1 is a mediator of osmotic stress response and of nutrient-dependent repression of sexual differentiation in the fission yeast Schizosaccharomyces pombe . Mol Gen Genomics 269, 116–125 (2003). https://doi.org/10.1007/s00438-003-0820-8

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