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Construction of a BAC library of Rosa rugosa Thunb. and assembly of a contig spanning Rdr1, a gene that confers resistance to blackspot

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Abstract

A BAC library to serve as a general tool for the physical mapping and positional cloning of rose genes has been constructed from Rosa rugosa DNA. With 27,264 clones the library contains 5.2 genome equivalents. The library was used to assemble a contig of BAC clones spanning Rdr1, a locus that confers resistance to blackspot. For this purpose fine-scale mapping of the target locus was achieved by bulked segregant analysis using 816 AFLP primer combinations. The target region around Rdr1 comprises about 400 kb and is covered by a minimum of six BAC clones. Furthermore, the detection of at least five resistance gene analogs of the TIR-NBS-LRR family on the contig indicates the presence of a cluster of resistance genes around Rdr1. These results will not only allow the isolation and identification of Rdr1 in the near future, but also provide the tools for the physical mapping and positional cloning of other horticulturally interesting genes in roses.

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Acknowledgements

We thank M. Schreiber and G. Bräcker for technical assistance, and Dr. A. Dohm for providing the colchicine-treated R. rugosa clone C5-23. We also thank Prof. A. Roberts and Dr. T. Lahaye for their critical review of this manuscript. The project was supported in part by grants from the Bundesanstalt für Landwirtschaft und Ernährung (No. 97HS043). All experiments described in this paper comply with current laws governing genetic experimentation in Germany

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Authors and Affiliations

  1. Institute of General Botany and Botanical Garden, Applied Molecular Biology of Plants (AMP) II, University of Hamburg, Ohnhorststrasse 18, 22609 , Hamburg, Germany

    H. Kaufmann & H. Lörz

  2. Institute of Ornamental Plant Breeding, Federal Centre for Breeding Research on Cultivated Plants, Bornkampsweg 31, 22926 , Ahrensburg, Germany

    L. Mattiesch & T. Debener

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  1. H. Kaufmann
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  2. L. Mattiesch
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  3. H. Lörz
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  4. T. Debener
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Correspondence to T. Debener.

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Communicated by R. Hagemann

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Kaufmann, H., Mattiesch, L., Lörz, H. et al. Construction of a BAC library of Rosa rugosa Thunb. and assembly of a contig spanning Rdr1, a gene that confers resistance to blackspot. Mol Gen Genomics 268, 666–674 (2003). https://doi.org/10.1007/s00438-002-0784-0

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  • DOI: https://doi.org/10.1007/s00438-002-0784-0

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