Abstract
Nuclei from Echinococcus granulosus protoscolices were isolated from infected sheep. Protein extracts were prepared for analysis of DNA-protein interactions involving specific transcriptional regulatory factors. Gel mobility-shift assays were done using a heterologous probe containing binding sites for widespread transcription factors. A fragment of the promoter of GATA-1 transcription factor from the chicken was selected. When nuclear extracts from E. granulosus protoscolices were assayed a specific band shift was observed. The methodologies developed in this study could provide an important contribution for the characterization of the DNA-protein interactions involved in transcriptional regulation within the context of recent developments in the molecular biology of this parasite.
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Received: 5 January 1998 / Accepted: 15 February 1998
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Garat, B., Esperón, P., Picón, M. et al. Echinococcus granulosus : preparation of protein extracts from protoscolex nuclei for mobility-shift assays. Parasitol Res 84, 598–600 (1998). https://doi.org/10.1007/s004360050457
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DOI: https://doi.org/10.1007/s004360050457