Abstract
The complete mitogenome (mtDNA) of nominal Paragonimus iloktsuenensis (Paragonimidae: Trematoda) and the nuclear ribosomal transcription unit (rTU) coding region (rTU*: from 5’-terminus of 18S to 3’-terminus of 28S rRNA gene, excluding the external spacer region) of this species and of P. ohirai were obtained and used to further support the previously suggested synonymy of these taxa in the P. ohirai complex. The complete mitogenome of P. iloktsuenensis was 14,827 bp long (GenBank: ON961029) and nearly identical to that of P. ohirai (14,818 bp; KX765277), with a 99.12% nucleotide identity. The rTU* was 7543 bp and 6932 bp in these two taxa, respectively. All genes and spacers in the rTU were identical in length, with exception of the first internal transcribed spacer, which contained multiple tandem repeat units (6.7 for P. iloktsuenensis and 5.7 for P. ohirai). There was near 100% identity for the rTU genes. The phylogenetic topology inferred from the mtDNA and from individual gene regions (partial cox1 of 387 bp and the ITS-2 of 282 bp − 285 bp) indicated a very close relationship consistent with synonymy of P. iloktsuenensis and P. ohirai. The datasets provided here will be useful for taxonomic reappraisal as well as studies of evolutionary and population genetics of the genus Paragonimus and family Paragonimidae.
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Data availability
The material and data obtained in this study are stored at the Institute of Biotechnology, Vietnam Academy of Science and Technology. The nucleotide sequences obtained in this study were submitted to GenBank under the accession numbers ON961029 for the mitogenome of P. iloktsuenensis, and OP081041 and OP081042 for the ribosomal transcription units of P. ohirai and P. iloktsuenensis, respectively.
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Funding
This research is funded by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 108.02–2020.07 (PI: Thanh Hoa Le).
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Thanh Hoa Le conceived and designed the study, analyzed the final data, prepared figures and tables, and wrote the manuscript. Khue Thi Nguyen, Linh Thi Khanh Pham, Huong Thi Thanh Doan, Do Thi Roan, and Xuyen Thi Kim Le conducted laboratory work and performed sequence analyses. Takeshi Agatsuma experimentally infected rodents, collected, molecularly identified, and provided adult specimens. David Blair reviewed all the data and drafts and finalized the MS. Takeshi Agatsuma, David Blair, and Thanh Hoa Le completed and approved the manuscript. The results were critically appraised and interpreted by all authors, who also read and approved the final manuscript version.
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Le, T.H., Nguyen, K.T., Pham, L.T.K. et al. Mitogenomic and nuclear ribosomal transcription unit datasets support the synonymy of Paragonimus iloktsuenensis and P. ohirai (Paragonimidae: Platyhelminthes). Parasitol Res 122, 1531–1544 (2023). https://doi.org/10.1007/s00436-023-07854-y
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DOI: https://doi.org/10.1007/s00436-023-07854-y