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Characterization and bio-efficacy of entomopathogenic Beauveria associated with cuticle-degrading enzymes to restrain sucking pest Bemisia tabaci

  • Immunology and Host-Parasite Interactions - Original Paper
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Abstract

The entomopathogenic Beauveria spp. were acquired from insect cadavers and soil rhizosphere of cotton, groundnut, and castor. Among Beauveria, five spp. derived from infected insects, eight Beauveria found from soil, and one strain of Beauveria bassiana collected from MTCC 9544. Beauveria were characterized for morphology and cuticle-degrading enzyme activity associated with virulence against Bemisia tabaci. The colony morphology, conidial arrangement, size, and shape confirmed all isolates as Beauveria. The chitinase (EC 3.2.1.14) and lipase (EC 3.1.1.3) activities were observed the highest in Beauveria JAU2, while higher protease (EC 3.4.21.4) activity found in JAU4 followed by JAU2 at 240 h. The bio-efficacy of Beauveria (1 × 107 conidia.ml−1) illustrated that potent JAU2 was examined with the highest % mortality and corrected mortality of B. tabaci at 144 h followed by JAU1. The LC90 and LC50were determined from potent (JAU1 and JAU2) and weak (JAU6), and it was found the lowest in JAU2. The most potent Beauveria JAU2, isolated from insect cadaver (Harmivora armigera), was illustrated higher virulence than other isolates. The Beauveria JAU2 were recognized as Beauveria bassiana based on the shape of conidia and size (2.00 to 2.09 µm dia) as examined in SEM. Study insight into recognition of potent Beauveria bassiana JAU2 was linked with cuticle-degrading enzyme activity for insecticidal action. The JAU2 isolate established the most positive correlation (P0.01: 0.864) between chitinase activity and corrected mortality of insect.

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  1. Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, 362 001, Gujarat, India

    Rushita V. Bhadani, H. P. Gajera, Darshna G. Hirpara & Harshita J. Kachhadiya

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  1. Rushita V. Bhadani
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  2. H. P. Gajera
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  3. Darshna G. Hirpara
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RVB carried out the experiment, microbial work, and biochemical analysis and working out the results; HPG was responsible for interpretation of data, writing of the manuscript, and liable for the idea and coordination of the experiment. DGH and HJK were responsible for helping in microbial and biochemical analysis. All authors contributed critically to the drafts and gave final approval for publication.

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Correspondence to H. P. Gajera.

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Bhadani, R.V., Gajera, H.P., Hirpara, D.G. et al. Characterization and bio-efficacy of entomopathogenic Beauveria associated with cuticle-degrading enzymes to restrain sucking pest Bemisia tabaci. Parasitol Res 121, 2019–2031 (2022). https://doi.org/10.1007/s00436-022-07557-w

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