Abstract
This study aimed to evaluate the effects of a commercial formulation containing fipronil and fluazuron on the reproductive biology and the morphology of ovaries from Rhipicephalus microplus engorged females. To carry out the study, three calves were artificially infested every 3 days with approximately 5000 larvae. On day 0, the animals were treated with a commercial formulation containing fipronil (1.25 mg/kg) + fluazuron (2.5 mg/kg). Before the application of the acaricide, engorged females of R. microplus were collected to constitute the control group (10 for biology analyses and 20 for histology analyses). After applying the commercial formulation, naturally detached engorged females were recovered on days + 5, + 10, and + 20 (10 engorged females/day) to evaluate their reproductive biology, and on days + 4, + 12, and + 20 (20 engorged females/day) for histological evaluation of the ovaries. Females from the treated groups produced smaller amounts of eggs, exhibiting lower viability when compared to eggs from the control group (p < 0.05). The ovaries of females from all treated groups (+ 4, + 12, and + 20) showed morphological changes, including: cytoplasmic disorganization, cytoplasmic degradation, irregular shape of the oocyte and germinal vesicle, reduction and vacuolization of yolk granules and oocyte disruption. Oocytes were observed in smaller numbers in all stages of development (I, II, III, IV, and V) and greater numbers of indeterminate oocytes were verified in the ovaries of the treated groups when compared to the control group. Therefore, results showed that the commercial formulation containing fipronil and fluazuron affected the reproductive biology, caused morphological changes in the ovaries, and reduced the number of oocytes in R. microplus engorged females.
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We thank the MSD Saúde Animal for the financial support, CNPq (National Council for Scientific and Technological Development) for the grants PQ (C.M.O. Monteiro—311889/2017–4 and Rocha T.L—306329/2020–4) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/CAPES—financial code 001, Brazil.
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Mirna Vieira Secchis: Student responsible for research development.
Letícia Vale: Execution of the research
Daniel de Castro Rodrigues: Execution of the research
Wendell Marcelo de Souza Perinotto: Study design and text review
Renata da Silva Matos: Execution of the research
Thiago Rocha Lopes: Study design, histological analyzes and text review
Pedro Vale de Azevedo Brito: Histological analyzes
Welber Daniel Zanetti Lopes: Study design and text review
Walquiria Arruda: Histological analyzes
Caio Márcio de Oliveira Monteiro: Study supervisor. Study design, writing and text review
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Secchis, M.V., Vale, L., de Castro Rodrigues, D. et al. Effect of the formulation with fipronil and fluazuron on the reproductive biology and ovaries histopathology of Rhipicephalus microplus engorged females. Parasitol Res 121, 839–849 (2022). https://doi.org/10.1007/s00436-022-07454-2
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DOI: https://doi.org/10.1007/s00436-022-07454-2